Jelinek T, Nothdurft H D, Löscher T
Department of Infectious Diseases and Tropical Medicine, University of Munich, Germany.
Trop Med Parasitol. 1995 Sep;46(3):154-7.
The objective of this investigation was to collect data concerning CS-antibody levels and duration of the immunological response to exposure of non-immune persons to a single malaria infection. For this purpose 156 specimens from 98 patients with confirmed falciparum malaria, 76 specimen derived from 64 patients with vivax malaria and sera from 32 patients who had not been previously to malarious areas were investigated by use of a commercially available ELISA testkit. All specimens from patients with falciparum malaria were also tested for merozoite-antibodies by an indirect fluorescence antibody test (IFAT). Positive levels of merozoite-antibodies were detectable in 89.1% of the specimen in this panel during the period between days 8 and 90 after onset of symptoms and decreased steadily thereafter. The test results were positive for CS-antibodies in 36.4% of the specimens from patients with falciparum malaria during the first 7 days after onset of symptoms. This figure increased to 55.8% during days 8-90 after onset and decreased to 38.9% in specimens which were tested later (91-1898 days). 11 specimens reacted positively to CS-antibody testing but negative in the IFAT. Therefore, the percentage of specimen detected by either IFAT or CS-ELISA was at 51.9% during days 0 and 7 (p < 0.001), 95.3% during days 8 and 90 (p = 0.039) and 44.4% for testing performed later (p < 0.001). CS-antibodies could also be detected in 5.3% of specimen from patients with vivax malaria while none of the sera from the malaria-negative control-group tested positive for CS-antibodies.(ABSTRACT TRUNCATED AT 250 WORDS)
本调查的目的是收集有关非免疫人群单次感染疟疾后CS抗体水平及免疫反应持续时间的数据。为此，使用市售ELISA检测试剂盒对98例确诊恶性疟患者的156份标本、64例间日疟患者的76份标本以及32例既往未去过疟疾流行区患者的血清进行了检测。所有恶性疟患者的标本还通过间接荧光抗体试验（IFAT）检测了裂殖子抗体。在症状出现后的第8天至90天期间，该组标本中有89.1%可检测到裂殖子抗体阳性水平，此后稳步下降。恶性疟患者发病后头7天，36.4%的标本CS抗体检测结果为阳性。发病后第8 - 90天，这一数字增至55.8%，而后期（91 - 1898天）检测的标本中该数字降至38.9%。11份标本CS抗体检测呈阳性，但IFAT检测呈阴性。因此，IFAT或CS - ELISA检测到的标本百分比在第0天和第7天为51.9%（p < 0.001），第8天和第90天为95.3%（p = 0.039），后期检测为44.4%（p < 0.001）。间日疟患者的标本中也有5.3%可检测到CS抗体，而疟疾阴性对照组的血清中CS抗体检测均为阴性。（摘要截选至25个字）
