Dudka J, Szczepaniak S
Katedra i Zakład Chemii Toksykologicznej Akademii Medycznej w Lublinie.
Rocz Panstw Zakl Hig. 1995;46(2):169-74.
The study was performed on 4 groups of male Wistar rats, receiving p.o. through 3 months every day: 1) sodium nitrite in dose 30 mg/kg b.w. x day (0.2 LD50); 2) copper chloride in dose 4.67 mg/kg b.w. x day (0.03 LD50); 3) copper chloride and sodium nitrite in amounts as above, and 4 - control group - received distilled water. The methemoglobin and hemoglobin were determined in whole blood and tryptophan in plasma 24 hours after the last intoxication. There was showed, that every day intoxication of rats with sodium nitrite cause the increase of methemoglobin concentration and decrease the free tryptophan level in the blood. There was also observed, that copper chloride, administrated together with sodium nitrite, decreases significantly his methemoglobin creating action.
该研究对4组雄性Wistar大鼠进行，连续3个月每天经口给予：1）剂量为30毫克/千克体重/天的亚硝酸钠（0.2 LD50）；2）剂量为4.67毫克/千克体重/天的氯化铜（0.03 LD50）；3）上述剂量的氯化铜和亚硝酸钠，以及4）对照组——给予蒸馏水。在最后一次中毒24小时后，测定全血中的高铁血红蛋白和血红蛋白以及血浆中的色氨酸。结果显示，大鼠每天摄入亚硝酸钠会导致高铁血红蛋白浓度升高，并降低血液中游离色氨酸水平。还观察到，与亚硝酸钠一起给予的氯化铜会显著降低其高铁血红蛋白生成作用。
