对来自矛头蝮蛇毒液的两种出血性金属蛋白酶BaH1和BaP1的免疫学研究。

Immunological studies on BaH1 and BaP1, two hemorrhagic metalloproteinases from the venom of the snake Bothrops asper.

作者信息

Rucavado A, Borkow G, Ovadia M, Gutiérrez J M

机构信息

Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.

出版信息

Toxicon. 1995 Aug;33(8):1103-6. doi: 10.1016/0041-0101(95)00039-o.

DOI:10.1016/0041-0101(95)00039-o

PMID:8533144

Abstract

No immunological cross-reactivity was observed between BaH1 and BaP1, two hemorrhagic metalloproteinases isolated from B. asper venom, by gel immunodiffusion, Western blotting and neutralization studies. Cross-reactivity was detected with antisera against these toxins in several crotaline and viperine snake venoms by ELISA, whereas no reactivity was observed with either antiserum against the venoms of Bothrops nummifer, Crotalus durissus terrificus, Vipera russelli and several elapid venoms. Antiserum against native BaH1 neutralized hemorrhagic activity of the venoms of B. asper, B. atrox, B. jararaca, Crotalus atrox, C. durissus durissus, Echis carinatus and Trimeresurus flavoviridis, being ineffective against the venoms of Agkistrodon bilineatus and Lachesis muta.

摘要

通过凝胶免疫扩散、蛋白质印迹法和中和研究发现，从糙鳞蝰蛇毒液中分离出的两种出血性金属蛋白酶BaH1和BaP1之间未观察到免疫交叉反应。通过酶联免疫吸附测定法（ELISA）检测到，几种响尾蛇科和蝰蛇科蛇毒的抗血清与这些毒素有交叉反应，而针对粗鳞矛头蝮、三色矛头蝮、罗素蝰蛇的毒液抗血清以及几种眼镜蛇科毒液抗血清均未观察到反应。针对天然BaH1的抗血清可中和糙鳞蝰蛇、矛头蝮、巴西矛头蝮、西部菱斑响尾蛇、杜氏响尾蛇、锯鳞蝰和竹叶青蛇毒液的出血活性，但对双带水蝮蛇和巨蝮蛇的毒液无效。