一种抗互变溶菌素-II单克隆抗体对具窍蝮蛇属和矛头蝮属蛇毒出血活性的中和作用

Neutralization of the hemorrhagic activity of Bothrops and Lachesis snake venoms by a monoclonal antibody against mutalysin-II.

作者信息

Estêvão-Costa M I, Martins M S, Sánchez E F, Diniz C R, Chávez-Olórtegui C

机构信息

Centro de Pesquisa e Desenvolvimento, Fundação Ezequiel Dias, Belo Horizonte, MG, Brazil.

出版信息

Toxicon. 2000 Jan;38(1):139-44. doi: 10.1016/s0041-0101(99)00134-8.

DOI:10.1016/s0041-0101(99)00134-8

PMID:10669019

Abstract

One mAb reactive with mutalysin-II, a hemorrhagic metalloproteinase isolated from Lachesis muta muta venom, was produced in mice immunized with L. m. muta venom. Indirect ELISA was employed to compare the antigenic cross-reactivity among the venoms from Bothrops snakes. The mAb anti-mutalysin-II efficiently neutralized the hemorrhagic effect of both mutalysin-II and L. m. muta crude venom. Furthermore, the mAb were cross-reactive with B. alternatus, B. atrox, B. itapetiningae, B. jararaca and B. neuwiedii and showed variable potencies in neutralizing the hemorrhagic activity of several bothropic venoms.

摘要

用矛头蝮蛇毒免疫小鼠，制备出一种与互溶素-II（一种从矛头蝮蛇毒中分离出的出血性金属蛋白酶）反应的单克隆抗体。采用间接酶联免疫吸附测定法比较了具窍蝮蛇属蛇毒之间的抗原交叉反应性。抗互溶素-II单克隆抗体有效地中和了互溶素-II和矛头蝮蛇粗毒的出血作用。此外，该单克隆抗体与黑腹具窍蝮蛇、矛头蝮、伊塔佩廷具窍蝮蛇、巴西具窍蝮蛇和纳氏具窍蝮蛇的蛇毒有交叉反应，并在中和几种具窍蝮蛇属蛇毒的出血活性方面表现出不同的效力。