Xu S, Shi F, Shen W, Lin J, Wang Y, Ye P, Tian E, Qian C, Lin B, Shi Y
Shanghai Institute of Animal Parasitology, Chinese Academy of Agricultural Sciences.
Vet Parasitol. 1995 Jul;58(4):301-12. doi: 10.1016/0304-4017(94)00735-u.
The protective potential of glutathione S-transferase (GST), keyhole limpet haemocyanin (KLH) and the freeze/thaw (F/T) schistosomula/BCG vaccine was evaluated against Schistosoma japonicum in the natural sheep host. Groups of ten sheep each were vaccinated as follows: Group I: 2 x F/T 30,000 schistosomula+BCG 3 x 10(8) organisms, with a 2 week interval between vaccinations (F/T 'Low'). Group II: 3 x F/T 20,000 schistosomula+BCG 3 x 10(8), with 4 week interval (F/T 'High'). Group III: 2 x GST 0.24 mg+FCA (Freund's complete adjuvant) with 2 week interval (GST 'Low'). Group IV: 3 x GST 0.24 mg+FCA, with 4 week interval (GST 'High'). Group V: 2 x KLH 1.0 mg in phosphate-buffered saline (PBS), with 2 week interval (KLH 'Low'). Group VI: 3 x KLH 1.0 mg in PBS, with 4 week interval (KLH 'High'). Group VII: control (not vaccinated). Specific antibody, detected by GST-enzyme-linked immunosorbent assay (ELISA) and KLH-ELISA on the day after the last vaccination and 1, 2 and 3 weeks post-challenge, was found in all GST- or KLH-vaccinated groups. The same was found in F/T schistosomula-vaccinated groups against crude adult worm antigen (AWA). In Western blotting all GST-vaccinated sera recognized 26 kDa and 28 kDa bands on the challenge day and at 3 and 11 weeks post-challenge. Mean faecal egg counts between Weeks 6 and 10 post-challenge were reduced in a statistically significant way at five time points in the four groups, i.e. 83.38% (P < 0.005) in Group II, 49.29% (P < 0.025) in Group III, 47.9% (P < 0.05) and 71.15% (P < 0.01) in Group IV, 52.0% (P < 0.025) and 66.38% (P < 0.025) in Group VI. On autopsy and perfusion 1 week after the last faecal count, adult worm reductions were obtained of 40.36% (P < 0.05) in Group I, 37.26% (P < 0.025) in Group II, 24.73% (not significant) in Group III, 35.93% (P < 0.025) in Group IV, 27.46% (P < 0.05) in Group V and 33.81% (P < 0.01) in Group VI. Mean tissue egg densities were also reduced significantly in Groups III, IV and VI, especially in Group IV vaccinated animals. Mean liver egg granuloma diameters of the vaccinated groups were found to be less than those of the controls but there was no statistical significance.(ABSTRACT TRUNCATED AT 400 WORDS)
在自然感染的绵羊宿主中，评估了谷胱甘肽S-转移酶（GST）、匙孔血蓝蛋白（KLH）以及冻融（F/T）尾蚴/卡介苗（BCG）疫苗对日本血吸虫的保护潜力。将绵羊分成每组10只的若干组，并按如下方式进行疫苗接种：第一组：2次接种F/T 30,000尾蚴 + BCG 3×10⁸个菌体，两次接种间隔2周（F/T“低剂量组”）。第二组：3次接种F/T 20,000尾蚴 + BCG 3×10⁸个菌体，间隔4周（F/T“高剂量组”）。第三组：2次接种GST 0.24毫克 + 弗氏完全佐剂（FCA），间隔2周（GST“低剂量组”）。第四组：3次接种GST 0.24毫克 + FCA，间隔4周（GST“高剂量组”）。第五组：2次接种含1.0毫克KLH的磷酸盐缓冲盐水（PBS）溶液，间隔2周（KLH“低剂量组”）。第六组：3次接种含1.0毫克KLH的PBS溶液，间隔4周（KLH“高剂量组”）。第七组：对照组（未接种）。在最后一次接种后的当天以及攻击感染后1、2和3周，通过GST酶联免疫吸附测定（ELISA）和KLH-ELISA检测发现，所有接种GST或KLH的组均产生了特异性抗体。接种F/T尾蚴的组针对成虫粗抗原（AWA）也出现了同样的情况。在蛋白质印迹法中，所有接种GST疫苗的血清在攻击感染当天以及攻击感染后3周和11周均识别出26 kDa和28 kDa的条带。在攻击感染后第6周至第10周期间，四组中的五个时间点的平均粪便虫卵计数均有统计学意义的降低，即第二组降低了83.38%（P < 0.005），第三组降低了49.29%（P < 0.025），第四组降低了47.9%（P < 0.05）和71.15%（P < 0.01），第六组降低了52.0%（P < 0.025）和66.38%（P < 0.025）。在最后一次粪便计数后1周进行尸检和灌注时，第一组成虫减少了40.36%（P < 0.05），第二组减少了37.26%（P < 0.025），第三组减少了24.73%（无统计学意义），第四组减少了35.93%（P < 0.025），第五组减少了27.46%（P < 0.05），第六组减少了33.81%（P < 0.01）。第三组、第四组和第六组的平均组织虫卵密度也显著降低，尤其是接种第四组疫苗的动物。接种组的平均肝虫卵肉芽肿直径小于对照组，但无统计学意义。（摘要截取自400字）
