Xu S, Shi F, Shen W, Lin J, Wang Y, Lin B, Qian C, Ye P, Fu L, Shi Y
Shanghai Institute of Animal Parasitology, Chinese Academy of Agricultural Sciences.
Vet Parasitol. 1993 Mar;47(1-2):37-50. doi: 10.1016/0304-4017(93)90174-l.
Four laboratory tests and one field trial with cryopreserved irradiated (CI) schistosomula vaccine and a freeze-thaw (F/T) vaccine against bovine Schistosomiasis japonica were carried out in 1979 and 1980 with the following results: (1) Single intradermal vaccination in buffalo calves each with 10,000 20 krad CI Schistosomula plus 1 ml BCG gave 62% worm reduction (P < 0.05). Using the same protocol 55% worm reduction (P < 0.01) was obtained in cattle. (2) Buffalo calves immunized twice, at a 1.5 month interval, with 10,000 and 20,000 CI schistosomula, respectively, resulted in a worm reduction 65%. (3) In a preliminary field trial with 10,000 CI schistosomula plus 1 ml BCG resulted in a worm reduction of 53% in buffalo calves. (4) Intradermal vaccination of 30,000 F/T schistosomula with 1 ml BCG was also tried in cattle and revealed a worm reduction of 57% but increasing the number of vaccinations did not improve the protective effect. (5) Evidence regarding the effects of immunization with CI vaccine in buffaloes and F/T vaccine in cattle, on the number of eggs and miracidia and that of female worms themselves was obtained. (6) Immune responses, cellular and humoral, elicited in buffaloes vaccinated with CI schistosomula were detected by means of Lymphocyte Transformation Assay and Enzyme Linked Immunosorbent Assay.
1979年和1980年进行了四项实验室试验和一项现场试验，试验对象是用于预防日本血吸虫病的冷冻辐照（CI）尾蚴疫苗和冻融（F/T）疫苗，结果如下：（1）在水牛犊牛中进行单次皮内接种，每头接种10,000条20千拉德的CI尾蚴加1毫升卡介苗，减虫率为62%（P<0.05）。采用相同方案，在牛中减虫率为55%（P<0.01）。（2）水牛犊牛分别间隔1.5个月接种两次，每次接种10,000条和20,000条CI尾蚴，减虫率为65%。（3）在一项初步现场试验中，给水牛犊牛接种10,000条CI尾蚴加1毫升卡介苗，减虫率为53%。（4）在牛中也尝试了皮内接种30,000条F/T尾蚴加1毫升卡介苗，减虫率为57%，但增加接种次数并未提高保护效果。（5）获得了关于CI疫苗对水牛和F/T疫苗对牛在虫卵、毛蚴数量以及雌虫本身数量影响的证据。（6）通过淋巴细胞转化试验和酶联免疫吸附试验检测了用CI尾蚴接种的水牛所引发的细胞免疫和体液免疫反应。
