Konno A, Hashimoto Y, Kon Y, Okada K, Davis W C, Sugimura M
Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Vet Immunol Immunopathol. 1995 Sep;48(1-2):105-12. doi: 10.1016/0165-2427(94)05412-l.
Histochemical characteristics and immunological surface phenotypes of globule leukocytes (GLs) of normal goats were investigated in the intestine. In the small intestine, GLs were concentrated in the base of the villus and around the crypt, whereas in the cecum and colon they were randomly distributed. Their cytoplasmic granules exclusively stained with phosphotungstic acid hematoxylin, and were negative for peroxidase and histamine in contrast to those of subepithelial mast cells. The existence of chondroitin sulfate in some granules of GLs and heparin in most granules of mast cells were revealed by alcian blue staining and digestion with chondroitinase ABC. Isolated intestinal GLs were positive for T cell receptor (TcR) 1-N24 (gamma delta) and CD8 alpha, and negative for WC1-N3 and WC1-N4. Cryostat sections of ileum revealed preferential intraepithelial distribution of both TcR1-N24+ cells and CD8+ cells. WC1-N3+ and WC1-N4+ cells were rarely seen in the epithelium and lamina propria. These results indicate that caprine GLs are a gamma delta T cell subset, which is a different cell population from WC1 positive gamma delta T cells.
对正常山羊肠道中的球状白细胞（GLs）的组织化学特征和免疫表面表型进行了研究。在小肠中，GLs集中在绒毛底部和隐窝周围，而在盲肠和结肠中它们随机分布。与上皮下肥大细胞相比，它们的细胞质颗粒仅用磷钨酸苏木精染色，而过氧化物酶和组胺呈阴性。通过阿尔辛蓝染色和用软骨素酶ABC消化，发现GLs的一些颗粒中存在硫酸软骨素，而肥大细胞的大多数颗粒中存在肝素。分离的肠道GLs对T细胞受体（TcR）1-N24（γδ）和CD8α呈阳性，对WC1-N3和WC1-N4呈阴性。回肠的冷冻切片显示TcR1-N24+细胞和CD8+细胞优先在上皮内分布。WC1-N3+和WC1-N4+细胞在上皮和固有层中很少见。这些结果表明，山羊GLs是一个γδ T细胞亚群，它是与WC1阳性γδ T细胞不同的细胞群体。
