Arneborg N, Høy C E, Jørgensen O B
Department of Biotechnology, Technical University of Denmark, Lyngby, Denmark.
Yeast. 1995 Aug;11(10):953-9. doi: 10.1002/yea.320111006.
The effects of produced ethanol and specific growth rate on the lipid content and composition of Saccharomyces cerevisiae CBS 2806 were studied using anaerobic chemostat cultures. The cells adapted to increased concentrations of produced ethanol by increasing the proportion of ergosterol at the expense of lanosterol, by increasing the proportion of phosphatidylinositol at the expense of phosphatidylcholine, and by increasing the amount of C18:0 fatty acids in total phospholipids at the expense of C16:0 fatty acids. The produced ethanol had no effect on the phospholipid content nor on the proportion of unsaturated fatty acids in the phospholipids. The specific growth rate had no effect on the phospholipid content, the sterol composition, the phospholipid composition, the fatty acid composition of total phospholipids, or on the proportion of unsaturated fatty acids in the phospholipids of S. cerevisiae. It was not possible to separate the effects of produced ethanol and growth rate on the ergosterol content of the chemostat-grown S. cerevisiae cells.
使用厌氧恒化器培养研究了所产生的乙醇和比生长速率对酿酒酵母CBS 2806脂质含量和组成的影响。细胞通过增加麦角固醇的比例（以羊毛固醇为代价）、增加磷脂酰肌醇的比例（以磷脂酰胆碱为代价）以及增加总磷脂中C18:0脂肪酸的含量（以C16:0脂肪酸为代价）来适应所产生乙醇浓度的增加。所产生的乙醇对磷脂含量以及磷脂中不饱和脂肪酸的比例均无影响。比生长速率对酿酒酵母的磷脂含量、固醇组成、磷脂组成、总磷脂的脂肪酸组成或磷脂中不饱和脂肪酸的比例均无影响。无法区分所产生的乙醇和生长速率对恒化器培养的酿酒酵母细胞中麦角固醇含量的影响。
