Department of Chemistry and Biochemistry, School of Medicine, University of Rijeka, HR-51000, Rijeka, Croatia.
Folia Microbiol (Praha). 2011 May;56(3):215-24. doi: 10.1007/s12223-011-0040-2. Epub 2011 May 31.
Phospholipid (PL) and fatty acid (FA) compositions of the plasma membrane (PM), as well as the FA composition of the PM phosphatidylcholine (PtdCho) and phosphatidylethanolamine (PtdEtn) in the pure culture (zero generation) and the first three recycled generations of the bottom-fermenting brewer's yeast, have been determined. The PL composition differed markedly among the generations; in the zero generation, phosphatidylinositol (PtdIns) was the main PL, accounting for 27% of total PLs, followed by phosphatidic acid and PtdCho. In all recycled generations, the main PL was PtdCho with a marked increase in the first generation compared with the zero (32% and 20%, respectively), followed by PtdIns in the first and second generations. In the FA composition of the PM, 22 FAs were identified, ranging from C(10) to C(26). The compositions of the PM FAs, as well as those of PtdCho and PtdEtn, were characterised by a high preponderance of C(16) acids. Saturated FAs prevailed in the zero generation, whilst unsaturated prevailed in the first and second generation. Although the profiles of FAs in PtdCho and PtdEtn were similar, some marked differences were observed, pointing out to their specific functions in the regulation of membrane properties.
已经测定了底层发酵啤酒酵母纯培养（零代）和前三代的质膜（PM）的磷脂（PL）和脂肪酸（FA）组成，以及 PM 磷脂酰胆碱（PtdCho）和磷脂酰乙醇胺（PtdEtn）的 FA 组成。各代之间的 PL 组成差异显著；在零代中，磷脂酰肌醇（PtdIns）是主要的 PL，占总 PL 的 27%，其次是磷脂酸和 PtdCho。在所有再生代中，主要的 PL 是 PtdCho，第一代与零代相比明显增加（分别为 32%和 20%），其次是第一代和第二代的 PtdIns。在 PM 的 FA 组成中，共鉴定出 22 种 FA，范围从 C(10)到 C(26)。PM FAs 的组成，以及 PtdCho 和 PtdEtn 的组成，以 C(16)酸的高优势为特征。零代中以饱和 FA 为主，第一代和第二代中以不饱和 FA 为主。尽管 PtdCho 和 PtdEtn 中的 FA 谱相似，但观察到一些明显的差异，表明它们在调节膜性质方面具有特定的功能。
