Trevithick J R, Dzialoszynski T
Department of Biochemistry, Faculty of Medicine, University of Western Ontario, London, Canada.
Biochem Mol Biol Int. 1994 Aug;33(6):1179-90.
A novel technique is reported which makes use of (1) an improved method for solubilizing luminol at neutral pH along with (2) the addition of dimethyl sulfoxide to stabilize superoxide anion in the solutions in which luminescence is detected. These improvements resulted in (1) very low blank values of luminescence and (2) an approximately 6-fold increase in sensitivity of detection of peroxide and superoxide, as well as stabilizing the superoxide radical anion. The technique can also be used to evaluate the availability of antioxidants in biological homogenates and fluids.
报道了一种新技术，该技术利用（1）一种在中性pH下溶解鲁米诺的改进方法，以及（2）添加二甲基亚砜来稳定检测发光的溶液中的超氧阴离子。这些改进带来了（1）极低的发光空白值，以及（2）过氧化物和超氧的检测灵敏度提高约6倍，同时稳定了超氧自由基阴离子。该技术还可用于评估生物匀浆和液体中抗氧化剂的有效性。
