Callery P S, Leslie J
J Pharm Sci. 1977 Apr;66(4):578-80. doi: 10.1002/jps.2600660430.
Extensive degradation of the 1,3-dimethyl derivative of phenobarbital occurs when it is injected with trimethylanilinium hydroxide in methanol into a gas chromatograph. The application of mass spectrometry demonstrated that the major products are N-methyl-2-phenylbutyramide, N,N-dimethyl-2-phenylbutyramide, methyl 2-phenylbutyrate, N,N,N'-trimethylethylphenylmalondiamide, and N,N,N',N'-tetramethylethylphenylmalondiamide. The methyl group of the ester arises from the methanol solvent, whereas the methyl groups of the substituted amides arise from the parent compound or the methylating reagent. Tetramethylammonium hydroxide causes much more degradation than trimethylanilinium hydroxide under comparable conditions.
当将苯巴比妥的1,3 - 二甲基衍生物与氢氧化三甲基苯胺在甲醇中注入气相色谱仪时，会发生广泛降解。质谱分析表明，主要产物是N - 甲基 - 2 - 苯基丁酰胺、N,N - 二甲基 - 2 - 苯基丁酰胺、2 - 苯基丁酸甲酯、N,N,N'- 三甲基乙基苯基丙二酰胺和N,N,N',N'- 四甲基乙基苯基丙二酰胺。酯的甲基来自甲醇溶剂，而取代酰胺的甲基来自母体化合物或甲基化试剂。在可比条件下，氢氧化四甲铵比氢氧化三甲基苯胺引起的降解要多得多。
