Ovalle R
Department of Natural Sciences, Hostos Community College, Bronx, New York 10451, USA.
Anal Biochem. 1995 Jul 20;229(1):1-7. doi: 10.1006/abio.1995.1370.
A potato homogenate containing proteins of similar molecular weights but varying pI values was separated by nonideal size-exclusion chromatography after attempts to resolve the proteins with common size-exclusion chromatography methods failed. The method used a zwitterion buffer to amplify electrostatic interactions between the proteins and the silica matrix. The proteins were sorted on the basis of pI in preference to molecular weight. Subsequent tests with marker proteins of known weight and pI verified the validity of the original findings. The method can be used for separation of isozymes and similarly sized proteins as an alternative to chromatofocusing or isoelectrofocusing.
在用常规尺寸排阻色谱法分离分子量相近但等电点（pI）值不同的马铃薯匀浆蛋白失败后，采用非理想尺寸排阻色谱法进行了分离。该方法使用两性离子缓冲液来增强蛋白质与硅胶基质之间的静电相互作用。蛋白质根据等电点而非分子量进行分类。随后用已知分子量和等电点的标记蛋白进行的测试验证了原始发现的有效性。该方法可用于分离同工酶和大小相似的蛋白质，作为色谱聚焦或等电聚焦的替代方法。
