Jeon S, Kim N, Hwang E, Hong S, Min Y G
Dept of Otolaryngology, GyeongSang National University Hospital, Chinju, Korea.
Ann Otol Rhinol Laryngol. 1995 Nov;104(11):895-8. doi: 10.1177/000348949510401112.
To investigate the physiological role of substance P innervation (SPI) in the nasal mucosa from inhaled irritant macromolecules, SPI in the rat nasal mucosa was selectively stimulated with capsaicin and the degree of horseradish peroxidase (HRP) permeability across the nasal epithelium was evaluated by enzyme immunoassay or by histochemical electron microscopy. The serum level of HRP was reduced significantly in capsaicin-administered rats compared to control rats along the time course with quantitative analysis of the enzyme immunoassay. Control rats showed heavy permeation of HRP across the epithelium, but capsaicin-administered rats showed weak permeation of HRP across the epithelium with histochemical electron microscopy. The epithelial lining and its tight junctions were left intact as judged by electron microscopy. In conclusion, selective stimulation of SPI of the rat nasal mucosa with capsaicin decreased the epithelial absorption permeability to HRP without compromising the epithelial integrity. These findings imply that the physiological role of capsaicin-sensitive SPI in the rat mucosa is to protect the airway from inhaled irritant macromolecules.
为了研究吸入性刺激性大分子物质在鼻黏膜中P物质神经支配（SPI）的生理作用，用辣椒素选择性刺激大鼠鼻黏膜中的SPI，并通过酶免疫测定法或组织化学电子显微镜评估辣根过氧化物酶（HRP）穿过鼻上皮的通透程度。通过酶免疫测定法的定量分析，与对照大鼠相比，辣椒素处理的大鼠血清中HRP水平在整个时间过程中显著降低。对照大鼠显示HRP大量透过上皮，但通过组织化学电子显微镜观察，辣椒素处理的大鼠显示HRP透过上皮的程度较弱。通过电子显微镜判断，上皮衬里及其紧密连接保持完整。总之，用辣椒素选择性刺激大鼠鼻黏膜的SPI可降低上皮对HRP的吸收通透性，而不损害上皮的完整性。这些发现表明，大鼠黏膜中对辣椒素敏感的SPI的生理作用是保护气道免受吸入性刺激性大分子物质的侵害。
