Petersson G, Svensjö E
Department of Oto-rhino-laryngology, University of Lund, Sweden.
Int J Microcirc Clin Exp. 1990 May;9(2):205-12.
Nasal permeability was studied in anaesthetized rats after topical challenge with methacholine (MC), substance P (SP), or capsaicin. The drugs were applied onto strips of filter paper. After drying, they were inserted into one side of the nasal cavity while the other side received strips without drugs. The filter paper strips were used not only to stimulate secretion but also to collect the resulting fluid. FITC-albumin was given i.v. and the amount appearing in the nasal fluid was captured on the strips and measured by fluorometry. The presence of FITC-albumin in nasal secretions reflects a leakage of plasma through the capillary endothelium and the nasal epithelium. MC, SP, and capsaicin stimulated the secretion of nasal fluid. SP and capsaicin, but not MC increased the concentration of FITC-albumin in the nasal fluid.
在麻醉大鼠中，用乙酰甲胆碱（MC）、P物质（SP）或辣椒素进行局部激发后，研究鼻通透性。将药物涂在滤纸条上。干燥后，将其插入鼻腔一侧，而另一侧插入不含药物的纸条。滤纸条不仅用于刺激分泌，还用于收集产生的液体。静脉注射异硫氰酸荧光素标记的白蛋白（FITC-白蛋白），出现在鼻液中的量被捕获在纸条上，并通过荧光测定法进行测量。鼻分泌物中FITC-白蛋白的存在反映了血浆通过毛细血管内皮和鼻上皮的渗漏。MC、SP和辣椒素刺激鼻液分泌。SP和辣椒素，但不是MC，增加了鼻液中FITC-白蛋白的浓度。
