通过聚合酶链反应检测活的微小隐孢子虫卵囊
Detection of viable Cryptosporidium parvum oocysts by PCR.
作者信息
Wagner-Wiening C, Kimmig P
机构信息
Landesgesundheitsamt Baden-Württemberg, Stuttgart, Germany.
出版信息
Appl Environ Microbiol. 1995 Dec;61(12):4514-6. doi: 10.1128/aem.61.12.4514-4516.1995.
Abstract
PCR was used to detect and specifically identify a gene fragment from Cryptosporidium parvum. An 873-bp region of a 2,359-bp DNA fragment encoding a repetitive oocyst protein of C. parvum was shown to be specifically amplified in C. parvum. An excystation protocol before DNA extraction allowed the differentiation between live and dead Cryptosporidium parvum oocysts.
摘要
采用聚合酶链反应（PCR）检测并特异性鉴定微小隐孢子虫的一个基因片段。微小隐孢子虫编码一种重复卵囊蛋白的2359 bp DNA片段中的一个873 bp区域在微小隐孢子虫中被特异性扩增。在DNA提取前采用的脱囊方案能够区分活的和死的微小隐孢子虫卵囊。
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