Wagner C, Kimmig P
Landesgesundheitsamt Baden-Württemberg, Stuttgart.
Gesundheitswesen. 1992 Nov;54(11):662-5.
Cryptosporidium parvum and Giardia lamblia are protozoan parasites which can cause gastroenteritis in man and animal. Surface water and drinking-water play an important role in transmission of the disease. The parasites can be detected by extensive and time-consuming procedures only. In this paper we describe a new way to isolate C. parvum and G. lamblia from water by a modification of essential steps of the original method of Rose. The new method applies the separation by flow centrifugation, the purification by different filters and the parasite isolation by percoll density gradient centrifugation. The modified method allows reproducible and by 20% increased recovery rates of cysts. Due to this fast and easy technique this procedure can be integrated in routine examinations.
微小隐孢子虫和蓝氏贾第鞭毛虫是可导致人和动物患肠胃炎的原生动物寄生虫。地表水和饮用水在该疾病的传播中起着重要作用。目前只能通过繁琐且耗时的程序检测这些寄生虫。在本文中，我们描述了一种通过改进Rose原始方法的关键步骤，从水中分离微小隐孢子虫和蓝氏贾第鞭毛虫的新方法。新方法采用流式离心分离、不同滤器纯化以及通过Percoll密度梯度离心进行寄生虫分离。改进后的方法使包囊回收率具有可重复性且提高了20%。由于这项快速简便的技术，该程序可纳入常规检查。
