Juggi J S, Abdulla A G, Bhatia K S, Ghaaedi F K, Makdisi Y, Mathew X
Department of Physiology, Faculty of Medicine, Kuwait University, Safat, Kuwait.
Basic Res Cardiol. 1995 Jul-Aug;90(4):314-22. doi: 10.1007/BF00797909.
Spontaneous diastolic myofilament motion of the isolated ischemic-reperfused rat heart was studied by the technique of laser spectroscopy. Scattered light intensity fluctuations (SLIF) from the exposed surface of the left ventricle of the quiescent perfused (Langendorff) rat heart were quantified by the autocorrelation function (R1F: frequency weighted by power), and by determining the average power of the SLIF signal (PS). The stabilized mean control (+/- SE) R1F (mV2/s2) and PS (mV2/s) values were: 0.87 +/- 0.07 and 21.3 +/- 1.5 respectively. SLIF were characterized to index the extent of cell Ca(2+)-loading and the integrity of the sarcoplasmic reticulum (SR) functions by low Na+ and ryanodine perfusions. Low Na+ perfusion significantly increased the R1F values and produced pronounced spectral peaks between 0.5 and 2.5 Hz frequency bands; whereas ryanodine (1 microM) perfusion completely abolished the SLIF signals. Ischemia (34 degrees C, 60 min.) produced nearly a 12-fold increase in the R1F and PS values accompanied by a four-fold increase in the left ventricular end-diastolic pressure (LVEDP) during the reperfusion period (34 degrees C, 30 min.) Pronounced reperfusion SLIF peaks were evident at the frequency bands between 0.25-5.0 Hz. Hypothermic (10 degrees C) preservation during ischemia reduced the frequency and the amplitude of the SLIF signals at various frequencies and prevented the rise in LVEDP. As compared to hypothermia alone, hypothermic cardioplegia offered a slightly better preservation. But hypothermia alone or in combination with cardioplegia failed to completely normalize the post-ischemic R1F and PS values.(ABSTRACT TRUNCATED AT 250 WORDS)
采用激光光谱技术研究了离体缺血再灌注大鼠心脏的自发性舒张期肌丝运动。通过自相关函数（R1F：功率加权频率）以及测定散射光强度波动（SLIF）信号的平均功率（PS），对静态灌注（Langendorff）大鼠心脏左心室暴露表面的散射光强度波动进行了量化。稳定的平均对照（±SE）R1F（mV2/s2）和PS（mV2/s）值分别为：0.87±0.07和21.3±1.5。通过低钠和雷诺丁灌注来表征SLIF，以指示细胞钙负荷程度和肌浆网（SR）功能的完整性。低钠灌注显著增加了R1F值，并在0.5至2.5 Hz频带产生明显的光谱峰；而雷诺丁（1 microM）灌注完全消除了SLIF信号。缺血（34℃，60分钟）使R1F和PS值增加近12倍，同时在再灌注期（34℃，30分钟）左心室舒张末期压力（LVEDP）增加四倍。在0.25 - 5.0 Hz频带明显出现再灌注SLIF峰。缺血期间低温（10℃）保存降低了不同频率下SLIF信号的频率和幅度，并防止了LVEDP升高。与单纯低温相比，低温停搏提供了稍好的保存效果。但单独低温或与停搏联合使用均未能使缺血后R1F和PS值完全恢复正常。（摘要截断于250字）
