Tiveljung A, Backström J, Forsum U, Monstein H J
Department of Clinical Microbiology, University Hospital, Linköping, Sweden.
APMIS. 1995 Oct;103(10):755-63. doi: 10.1111/j.1699-0463.1995.tb01434.x.
Using DNA primers based on highly conserved regions of bacterial 16S ribosomal RNA genes, a technique was established for detection of Mobiluncus species by polymerase chain reaction (PCR) and hybridization analysis. Part of the 16S rRNA genes of Mobiluncus mulieris, Mobiluncus curtisii and uncharacterized Mobiluncus strains were analyzed by broad-range PCR amplification and direct DNA sequencing analysis. Sequence comparison of the partial 16S rRNA genes of Mobiluncus curtisii, Mobiluncus mulieris and atypical Mobiluncus strains studied indicated genus and species-specific motifs within the variable regions V3, V4 and V9 of 16S ribosomal DNAs. A Mobiluncus curtisii-specific primer, located within the variable region V3 of the 16S rRNA gene, was designed for Southern blot hybridization analysis of broad-range PCR products. Broad-range amplification combined with a M. curtisii-specific hybridization probe, Mob V3, distinguished between Mobiluncus curtisii, Mobiluncus mulieris, and atypical Mobiluncus strains.
利用基于细菌16S核糖体RNA基因高度保守区域的DNA引物，建立了一种通过聚合酶链反应（PCR）和杂交分析检测动弯杆菌属菌种的技术。通过广谱PCR扩增和直接DNA测序分析，对多形动弯杆菌、柯氏动弯杆菌及未鉴定的动弯杆菌菌株的部分16S rRNA基因进行了分析。对所研究的柯氏动弯杆菌、多形动弯杆菌及非典型动弯杆菌菌株的部分16S rRNA基因进行序列比较，结果表明在16S核糖体DNA的可变区V3、V4和V9内存在属和种特异性基序。设计了一种位于16S rRNA基因可变区V3内的柯氏动弯杆菌特异性引物，用于对广谱PCR产物进行Southern印迹杂交分析。广谱扩增结合柯氏动弯杆菌特异性杂交探针Mob V3，可区分柯氏动弯杆菌、多形动弯杆菌和非典型动弯杆菌菌株。
