通过免疫印迹技术区分柯氏动弯杆菌和女性动弯杆菌的抗原谱。

Antigenic profiles for the differentiation of Mobiluncus curtisii and Mobiluncus mulieris by immunoblotting technique.

作者信息

Gatti M, Aschbacher R, Cimmino C, Valentini R

机构信息

Department of Clinical and Experimental Medicine, Faculty of Medicine, University of Bologna, Italy.

出版信息

New Microbiol. 1997 Jul;20(3):247-52.

PMID:9258945

Abstract

The antigenic profile of 30 vaginal isolates of Mobiluncus strains (22 M. curtisii and 8 M. mulieris) was determined by immunoblotting technique using mouse immune ascitic fluids containing polyclonal antibodies against the type strains M. curtisii subsp. holmesii (ATCC 35242) and M. mulieris (ATCC 35243). Two antigenic profiles were identified within M. curtisii isolates, whereas a certain variability was observed among M. mulieris strains where at least three antigens were constantly recognized. The detection of antigenic profiles of Mobiluncus strains by immunoblotting technique provided a simple method to identify Mobiluncus isolates at the species level.

摘要

采用免疫印迹技术，利用含有针对柯氏动弯杆菌亚种霍姆斯氏菌（ATCC 35242）和穆氏动弯杆菌（ATCC 35243）型菌株的多克隆抗体的小鼠免疫腹水，测定了30株阴道动弯杆菌菌株（22株柯氏动弯杆菌和8株穆氏动弯杆菌）的抗原谱。在柯氏动弯杆菌分离株中鉴定出两种抗原谱，而在穆氏动弯杆菌菌株中观察到一定的变异性，其中至少有三种抗原能持续被识别。通过免疫印迹技术检测动弯杆菌菌株的抗原谱，为在种水平上鉴定动弯杆菌分离株提供了一种简单方法。