Renz H, Bradley K, Gelfand E W
Department of Pediatrics, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado, USA.
Am J Respir Cell Mol Biol. 1996 Jan;14(1):36-43. doi: 10.1165/ajrcmb.14.1.8534484.
Sensitization of BALB/c mice to ovalbumin (OVA) through the airways stimulated allergen-specific immediate hypersensitivity responses and these effects were related to the expansion of V beta 8.1/8.2+ T cells. In contrast, splenic V beta 2+ T cells from sensitized animals inhibited V beta 8.1/8.2+ T-cell induction of anti-OVA IgE production in vivo. To examine whether such differences in T-cell function were associated with differences in cytokine production, CD4+ T cells and CD4+ T cells depleted of V beta 8.1/8.2+ T cells were analyzed for interleukin-4 (IL-4) and interferon-gamma (IFN-gamma) production. In nonsensitized animals, no differences in IL-4 and IFN-gamma production were found in mRNA levels as well as in protein levels in these two populations of cells. In contrast, CD4+ T cells from sensitized mice showed higher IL-4 and lower IFN-gamma production than CD4+ cells depleted of V beta 8+ lymphocytes. Similar results were obtained after stimulation of CD4+ T cells from OVA-sensitized animals with anti-V beta 2 and anti-V beta 8.1/8.2 antibodies. Stimulation of V beta 8.1/8.2+ T cells from sensitized mice with OVA or OVA peptide 323-339 also resulted in increased production of IL-4. These data indicate that allergen sensitization via the airways stimulates the selective expansion of certain V beta-expressing T cells and that these T-cell subsets exhibit different functional activities in terms of cytokine production.
通过气道使BALB/c小鼠对卵清蛋白（OVA）致敏，刺激了变应原特异性速发型超敏反应，这些效应与Vβ8.1/8.2⁺ T细胞的扩增有关。相比之下，致敏动物的脾脏Vβ2⁺ T细胞在体内抑制了Vβ8.1/8.2⁺ T细胞诱导的抗OVA IgE产生。为了研究T细胞功能的这种差异是否与细胞因子产生的差异相关，分析了CD4⁺ T细胞和去除Vβ8.1/8.2⁺ T细胞的CD4⁺ T细胞的白细胞介素-4（IL-4）和干扰素-γ（IFN-γ）产生情况。在未致敏动物中，这两种细胞群体在mRNA水平和蛋白质水平上的IL-4和IFN-γ产生均未发现差异。相比之下，致敏小鼠的CD4⁺ T细胞比去除Vβ8⁺淋巴细胞的CD4⁺细胞表现出更高的IL-4产生和更低的IFN-γ产生。用抗Vβ2和抗Vβ8.1/8.2抗体刺激OVA致敏动物的CD4⁺ T细胞后也得到了类似结果。用OVA或OVA肽323 - 339刺激致敏小鼠的Vβ8.1/8.2⁺ T细胞也导致IL-4产生增加。这些数据表明，通过气道进行变应原致敏刺激了某些表达Vβ的T细胞的选择性扩增，并且这些T细胞亚群在细胞因子产生方面表现出不同的功能活性。
