Hamelmann E, Oshiba A, Paluh J, Bradley K, Loader J, Potter T A, Larsen G L, Gelfand E W
Division of Basic Sciences, Department of Pediatrics, University of Colorado Health Science Center, Denver 80206, USA.
J Exp Med. 1996 Apr 1;183(4):1719-29. doi: 10.1084/jem.183.4.1719.
To study the role of CD8+ T cells in allergic sensitization, we examined the effects of in vivo depletion of CD8+ T cells prior to sensitization on IgE production, immediate type cutaneous hypersensitivity and development of altered airway responsiveness. BALB/c mice were thymectomized and treated with anti-CD8 antibody resulting in depletion of CD8+ T cells (<1%) in spleen and lymphoid tissues. In these mice, sensitization to ovalbumin (OVA) via the airways still resulted in IgE anti-OVA responses and immediate cutaneous reactions to OVA, but the animals were unable to develop airway hyperresponsiveness, eosinophil infiltration of the lung parenchyma, or IL-5 production in the local lymph nodes of the airway. Transfer of CD8+ T cells from naive animals during sensitization (on day 8 of the 10-d protocol) fully restored the ability to develop airway hyperresponsiveness and this was accompanied by IL-5 production and eosinophil accumulation in the lung. These data indicate a critical role for CD8+ T cells in the production of IL-5 and the development of altered airway responsiveness after antigen sensitization through the airways.
为研究CD8⁺ T细胞在变应性致敏中的作用，我们检测了致敏前体内清除CD8⁺ T细胞对IgE产生、速发型皮肤超敏反应及气道反应性改变发展的影响。对BALB/c小鼠进行胸腺切除并给予抗CD8抗体处理，导致脾脏和淋巴组织中CD8⁺ T细胞耗竭（<1%）。在这些小鼠中，经气道对卵清蛋白（OVA）致敏仍可导致IgE抗OVA反应及对OVA的速发型皮肤反应，但动物无法产生气道高反应性、肺实质嗜酸性粒细胞浸润或气道局部淋巴结中IL-5的产生。在致敏期间（10天方案的第8天）从未致敏动物转移CD8⁺ T细胞可完全恢复产生气道高反应性的能力，这伴随着IL-5的产生和肺中嗜酸性粒细胞的聚集。这些数据表明，CD8⁺ T细胞在经气道抗原致敏后IL-5的产生及气道反应性改变的发展中起关键作用。
