Kistler J, Evans C, Donaldson P, Bullivant S, Bond J, Eastwood S, Roos M, Dong Y, Gruijters T, Engel A
Department of Biochemistry and Molecular Biology, School of Biological Sciences, University of Auckland, New Zealand.
Microsc Res Tech. 1995 Aug 1;31(5):347-56. doi: 10.1002/jemt.1070310504.
Recent advances in understanding lens fiber gap junction formation are reviewed. These include studies of junctional protein expression in the embryonic lens, and of age related changes affecting gap junction structure and composition in the adult lens. An in vitro assembly system based on detergent solubilized pore complexes and endogenous lipids has been developed to provide information on the molecular interactions involved in gap junction formation and to provide material for structure analysis. Important information on the electrical properties of lens gap junction channels is obtained using electrophysiological techniques including planar lipid bilayer analysis and patch clamping.
本文综述了晶状体纤维间隙连接形成方面的最新进展。这些进展包括对胚胎晶状体中连接蛋白表达的研究，以及对影响成年晶状体间隙连接结构和组成的年龄相关变化的研究。已开发出一种基于去污剂溶解的孔复合物和内源性脂质的体外组装系统，以提供有关间隙连接形成中分子相互作用的信息，并为结构分析提供材料。使用包括平面脂质双层分析和膜片钳在内的电生理技术，获得了有关晶状体间隙连接通道电特性的重要信息。
