Jiang P P, Hansen T H, Shreffler D C, Miller R D
Department of Genetics, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Mamm Genome. 1995 Sep;6(9):586-91. doi: 10.1007/BF00352362.
In this study we exploit the unique genetic resource of inbred mouse major histocompatibility complex (H2) congenic and recombinant strains to construct a high-resolution map of microsatellite loci in and around the H2 region, as well as an independent genetic map of other loci on mouse Chromosome (Chr) 17. Microsatellite loci were analyzed in 11 C57BL/10 (B10) strains to determine the size of the congenic interval in each. The length of the congenic interval found in each strain varied widely. Interestingly, the intervals were generally smaller than statistical expectations. However, the observed congenic intervals were still sufficiently long that these strains and probably wild-derived H2 congenics are an important source of genetic variability. The staggered ends of the various congenic intervals and the recombinants were used to construct the map. This map will be useful for physical cloning and to help localize novel genes. As evidence of the mapping application of congenic strains, locational information was derived about Trp53-ps and Stl.
在本研究中，我们利用近交系小鼠主要组织相容性复合体（H2）同类系和重组品系的独特遗传资源，构建H2区域及其周围微卫星位点的高分辨率图谱，以及小鼠第17号染色体（Chr）上其他位点的独立遗传图谱。对11个C57BL/10（B10）品系中的微卫星位点进行了分析，以确定每个品系中同类系区间的大小。在每个品系中发现的同类系区间长度差异很大。有趣的是，这些区间通常小于统计预期。然而，观察到的同类系区间仍然足够长，以至于这些品系以及可能源自野生的H2同类系是遗传变异的重要来源。利用各种同类系区间和重组体的交错末端构建图谱。该图谱将有助于物理克隆并有助于定位新基因。作为同类系品系图谱应用的证据，获得了关于Trp53-ps和Stl的定位信息。
