Roman S, Moldovan I, Călugăru A, Regalia T, Sulică A
Center of Immunology, St. Nicolau Institute of Virology, Bucharest, Romania.
Rom J Intern Med. 1995 Jan-Jun;33(1-2):27-36.
The paper reports the distribution of lymphocyte subpopulations in the Caucasian population of Romania. Investigations were carried out in cells bearing the following antigens: CD3 (T cells), CD19 (B cells), CD4 (T helper/inducer cells), CD8 (T suppressor/cytotoxic and some NK cells), and CD16 CD56 (NK cells). Reference values for the lymphocyte subpopulation were obtained from over 100 healthy Caucasian adult volunteers. Blood from these donors was analyzed using FACScan flow cytometer, Leuco-GATE, Simultest and FACS Lysing Solution, and SimulSET software. As an internal quality control, it was verified that %T+%B+%NK approximates 100% in all samples. The results presented here, obtained on healthy donors (51 males and 49 females), showed that there are no statistically significant variations of CD4/CD8 ratio related to sex or age, the mean value of this ratio (2.0 +/- 0.02) being similar to that reported by the West-European countries. Additional similarities were found when the relative percentage, mean +/- standard deviation (CD3 = 74.2 +/- 2.8; CD19 = 10.8 +/- 1.6; CD4 = 42.0 +/- 2.5; CD8 = 28.9 +/- 5.7; CD56 = 15.2 +/- 0.4) and the absolute cell number of the major peripheral blood mononuclear subsets established in our study were compared with other published results. This study was entirely supported by Becton Dickinson--Europe (Division Heidelberg, Germany).
该论文报道了罗马尼亚白种人群中淋巴细胞亚群的分布情况。研究针对携带以下抗原的细胞展开：CD3（T细胞）、CD19（B细胞）、CD4（T辅助/诱导细胞）、CD8（T抑制/细胞毒性细胞及部分自然杀伤细胞）以及CD16 CD56（自然杀伤细胞）。淋巴细胞亚群的参考值取自100多名健康的白种成年志愿者。使用FACScan流式细胞仪、Leuco - GATE、Simultest和FACS Lysing Solution以及SimulSET软件对这些供体的血液进行分析。作为内部质量控制，在所有样本中均验证了%T + %B + %NK约等于100%。此处呈现的结果是在健康供体（51名男性和49名女性）上获得的，结果表明，CD4/CD8比值在性别或年龄方面不存在统计学上的显著差异，该比值的平均值（2.0 +/- 0.02）与西欧国家报道的相似。当将我们研究中确定的主要外周血单核细胞亚群的相对百分比、平均值 +/- 标准差（CD3 = 74.2 +/- 2.8；CD19 = 10.8 +/- 1.6；CD4 = 42.0 +/- 2.5；CD8 = 28.9 +/- 5.7；CD56 = 15.2 +/- 0.4）以及绝对细胞数与其他已发表的结果进行比较时，发现了更多相似之处。本研究完全由德国海德堡分公司的贝克顿·迪金森欧洲公司资助。
