采用纤维素酶消化和光谱法对仙客来种子木葡聚糖寡糖进行结构分析。

Structural analysis of cyclamen seed xyloglucan oligosaccharides using cellulase digestion and spectroscopic methods.

作者信息

Braccini I, Hervé du Penhoat C, Michon V, Goldberg R, Clochard M, Jarvis M C, Huang Z H, Gage D A

机构信息

Département de Chimie, U.R.A. 1679, E.N.S., Paris, France.

出版信息

Carbohydr Res. 1995 Oct 16;276(1):167-81. doi: 10.1016/0008-6215(95)00156-n.

DOI:10.1016/0008-6215(95)00156-n

PMID:8536253

Abstract

Xyloglucan polymers have been isolated from cyclamen seeds and characterized by both liquid and CP-MAS 13C NMR spectroscopy. Treatment of the polysaccharides with cellulase from Trichoderma viride afforded XG oligomers which have been studied with both mass spectrometry and NMR spectroscopy. The repeating unit in the intact polymers and the most abundant hydrolysis product correspond to the Gal1 Xyl3 Glc4 (XXLG) fragment. However, detection of notable amounts of Xyl3 Glc4 (XXXG) and Gal2 Xyl3 Glc4 (XLLG) indicates that the galactose distribution in xyloglucan from cyclamen is irregular. FAB-MS analysis of a new derivative, prepared by forming the glycosamine of m-tetrafluoroethoxy aniline, has led to unambiguous sequence information for the XXLG oligomer.

摘要

木葡聚糖聚合物已从仙客来种子中分离出来，并通过液体和交叉极化魔角旋转13C核磁共振光谱进行了表征。用绿色木霉的纤维素酶处理多糖得到了XG低聚物，并用质谱和核磁共振光谱对其进行了研究。完整聚合物中的重复单元和最丰富的水解产物对应于Gal1 Xyl3 Glc4（XXLG）片段。然而，检测到大量的Xyl3 Glc4（XXXG）和Gal2 Xyl3 Glc4（XLLG）表明仙客来木葡聚糖中的半乳糖分布是不规则的。通过形成间四氟乙氧基苯胺的糖胺制备的一种新衍生物的快原子轰击质谱分析，得到了XXLG低聚物明确的序列信息。