O'Broin S D, Kelleher B P, Gunter E
Department of Haematology, St James's Hospital, Dublin 8, Ireland.
Clin Lab Haematol. 1995 Jun;17(2):185-8.
Dried blood spots (DBS) on filter paper have potential as a collection method in screening for haematinic deficiencies. Factors influencing the volumetric precision of uniform 'centre' punches (6.35 mm diameter) removed from dried blood spots have been evaluated. The volume of blood in each DBS punch (n = 234) was greatly influenced by both sample haematocrit (r = 0.63) and haemoglobin concentration (r = 0.63). The volume in identical punches (n = 57) also differed significantly when measured independently using either 125I human serum albumin or haemoglobin relative to the original blood sample. DBS punch volumes should be predetermined for individual batches of filter paper using the specific analyte of interest.
滤纸上的干血斑（DBS）有潜力作为筛查造血物质缺乏症的一种采集方法。已对影响从干血斑上取下的均匀“中心”冲孔（直径6.35毫米）体积精度的因素进行了评估。每个DBS冲孔（n = 234）中的血量受样本血细胞比容（r = 0.63）和血红蛋白浓度（r = 0.63）的影响很大。当使用¹²⁵I人血清白蛋白或血红蛋白相对于原始血样独立测量时，相同冲孔（n = 57）中的体积也存在显著差异。应使用感兴趣的特定分析物针对各批滤纸预先确定DBS冲孔体积。
