Braissant O, Foufelle F, Scotto C, Dauça M, Wahli W
Institut de Biologie Animale, Bâtiment de Biologie, Université de Lausanne, Switzerland.
Endocrinology. 1996 Jan;137(1):354-66. doi: 10.1210/endo.137.1.8536636.
Peroxisome proliferator-activated receptors (PPARs) are members of the nuclear hormone receptor superfamily that can be activated by various xenobiotics and natural fatty acids. These transcription factors primarily regulate genes involved in lipid metabolism and also play a role in adipocyte differentiation. We present the expression patterns of the PPAR subtypes in the adult rat, determined by in situ hybridization using specific probes for PPAR-alpha, -beta and -gamma, and by immunohistochemistry using a polyclonal antibody that recognizes the three rat PPAR subtypes. In numerous cell types from either ectodermal, mesodermal, or endodermal origin, PPARs are coexpressed, with relative levels varying between them from one cell type to the other. PPAR-alpha is highly expressed in hepatocytes, cardiomyocytes, enterocytes, and the proximal tubule cells of kidney. PPAR-beta is expressed ubiquitously and often at higher levels than PPAR-alpha and -gamma. PPAR-gamma is expressed predominantly in adipose tissue and the immune system. Our results suggest new potential directions to investigate the functions of the different PPAR subtypes.
过氧化物酶体增殖物激活受体（PPARs）是核激素受体超家族的成员，可被多种外源性物质和天然脂肪酸激活。这些转录因子主要调节参与脂质代谢的基因，并且在脂肪细胞分化中也发挥作用。我们通过使用针对PPAR-α、-β和-γ的特异性探针进行原位杂交，以及使用识别三种大鼠PPAR亚型的多克隆抗体进行免疫组织化学，展示了成年大鼠中PPAR亚型的表达模式。在源自外胚层、中胚层或内胚层的多种细胞类型中，PPARs共同表达，它们之间的相对水平因细胞类型而异。PPAR-α在肝细胞、心肌细胞、肠上皮细胞和肾近端小管细胞中高度表达。PPAR-β广泛表达，且其表达水平通常高于PPAR-α和-γ。PPAR-γ主要在脂肪组织和免疫系统中表达。我们的结果为研究不同PPAR亚型的功能提示了新的潜在方向。
