Eilertson C D, Kittilson J D, Sheridan M A
Department of Zoology, North Dakota State University, Fargo 58105, USA.
Gen Comp Endocrinol. 1995 Aug;99(2):211-20. doi: 10.1006/gcen.1995.1104.
Somatostatins are a diverse family of peptides known to modulate insulin and glucagon secretion as well as to stimulate glycogenolysis and lipolysis in salmonid fish. In this study, Brockmann bodies (bisected to yield hemi-islets) isolated from rainbow trout, Oncorhynchus mykiss, were used to study the effects of insulin, glucagon, and somatostatin at various concentrations of glucose (1, 5, and 10 mM) on pancreatic somatostatin release. The release of somatostatin-25, the most predominate form of somatostatin in salmonid pancreas, was stimulated by insulin in the presence of 1 and 5 mM glucose but not in the presence of 10 mM glucose, whereas glucagon stimulated somatostatin-25 release only in the presence of high (10 mM) glucose. Somatostatin-25 release also was stimulated by somatostatin-14. The secretion of somatostatin-14 was suppressed by insulin in the presence of 5 and 10 mM glucose and was stimulated by glucagon in the presence of high (10 mM) glucose. These results indicate that insulin, glucagon, and somatostatin-14 are regulators of somatostatin-14 and somatostatin-25 pancreatic release in rainbow trout and that these effects are modulated by glucose.
生长抑素是一类多样的肽家族，已知其可调节胰岛素和胰高血糖素的分泌，并刺激鲑科鱼类的糖原分解和脂肪分解。在本研究中，从虹鳟鱼（Oncorhynchus mykiss）分离出的布罗克曼体（切成两半得到半胰岛）用于研究在不同葡萄糖浓度（1、5和10 mM）下胰岛素、胰高血糖素和生长抑素对胰腺生长抑素释放的影响。生长抑素-25是鲑科胰腺中最主要的生长抑素形式，在1和5 mM葡萄糖存在时，胰岛素刺激其释放，但在10 mM葡萄糖存在时则不然，而胰高血糖素仅在高浓度（10 mM）葡萄糖存在时刺激生长抑素-25释放。生长抑素-14也刺激生长抑素-25的释放。在5和10 mM葡萄糖存在时，胰岛素抑制生长抑素-14的分泌，而在高浓度（10 mM）葡萄糖存在时，胰高血糖素刺激其分泌。这些结果表明，胰岛素、胰高血糖素和生长抑素-14是虹鳟鱼胰腺中生长抑素-14和生长抑素-25释放的调节因子，且这些作用受葡萄糖调节。
