Rosa C, Osborne S, Garetto F, Griva S, Rivella A, Calabresi G, Guaschino R, Bonelli F
Sorin Biomedica, R&D Diagnostic Division, Saluggia (VC), Italy.
J Virol Methods. 1995 Oct;55(2):219-32. doi: 10.1016/0166-0934(95)00060-8.
Specific domains of the NS4 and NS5 gene products of hepatitis C virus have been identified using hydrophilicity profiles for the prediction of potential immunogenic regions, and epitope scanning techniques. Peptides synthesised on the basis of such data show excellent reactivity in the ELISA format. Introduction of a glycine-glycine spacer between two peptides (NS4-12 and NS5-44) to give a single chimeric peptides does not appear to impair immunoreactivity. An ELISA based on the chimeric peptide and a Core-NS3 recombinant protein correctly diagnoses a cohort of haemodialysed patients, three commercial HCV panels and the sera of a negative control population.
利用亲水性图谱预测潜在免疫原性区域以及表位扫描技术，已确定了丙型肝炎病毒NS4和NS5基因产物的特定结构域。基于这些数据合成的肽在ELISA检测中显示出优异的反应性。在两种肽（NS4 - 12和NS5 - 44）之间引入甘氨酸 - 甘氨酸间隔区以形成单一嵌合肽，似乎并未损害免疫反应性。基于该嵌合肽和核心 - NS3重组蛋白的ELISA能够正确诊断一组血液透析患者、三种商用HCV检测试剂以及一个阴性对照人群的血清。
