Raiden S, Polack E, Nahmod V, Labeur M, Holsboer F, Arzt E
Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
J Clin Immunol. 1995 Sep;15(5):242-9. doi: 10.1007/BF01540881.
This work examined whether (1) immune cells express thyrotrophin releasing hormone (TRH) receptor mRNA and (2) TRH modulates lymphocyte activation. By Northern blot of RNA extracted from human peripheral blood mononuclear cells (PBMC) and rat splenocytes, a single TRH receptor mRNA band of about 3.8 kb (identical to that obtained from pituitary cells) was obtained, under both basal and stimulated conditions. A significant increase in DNA synthesis was observed in phytohemagglutinin-stimulated PBMC and concanavalin A (Con A) stimulated splenocytes when TRH (10(-6) M-10(-12) M) was added. After 5, 30, 60, 180 min and 24 h of TRH administration in vivo, a significant increase in the rat splenocyte proliferative response to Con A was observed. In vivo administration of anti-rat TSH antibody (1/1000) blocked the increase observed after 30 min of TRH administration on the Con A stimulated splenocyte response. TRH possess immunostimulatory functions directly via its receptor and indirectly via release of other immunostimulatory factors such as thyrotrophin.
（1）免疫细胞是否表达促甲状腺激素释放激素（TRH）受体mRNA；（2）TRH是否调节淋巴细胞激活。通过对从人外周血单核细胞（PBMC）和大鼠脾细胞中提取的RNA进行Northern印迹分析，在基础条件和刺激条件下，均获得了一条约3.8 kb的单一TRH受体mRNA条带（与从垂体细胞中获得的条带相同）。当加入TRH（10⁻⁶ M - 10⁻¹² M）时，在植物血凝素刺激的PBMC和刀豆蛋白A（Con A）刺激的脾细胞中观察到DNA合成显著增加。在体内给予TRH 5、30、60、180分钟和24小时后，观察到大鼠脾细胞对Con A的增殖反应显著增加。体内给予抗大鼠促甲状腺激素抗体（1/1000）可阻断TRH给药30分钟后观察到的Con A刺激的脾细胞反应的增加。TRH通过其受体直接以及通过释放其他免疫刺激因子（如促甲状腺激素）间接具有免疫刺激功能。
