Man Y G, Ball W D, Culp D J, Hand A R, Moreira J E
Department of Anatomy, Howard University, College of Medicine, Washington, DC 20059, USA.
J Histochem Cytochem. 1995 Dec;43(12):1203-15. doi: 10.1177/43.12.8537636.
In the perinatal submandibular gland (SMG) of the rat, Type I cells secrete protein C (89 KD) and Type III cells secrete B1-immunoreactive proteins (20-30 KD); both cell types secrete protein D (175 KD). After the disappearance of both perinatal cell types from the maturing acini, only cells of the intercalated ducts (ID) show strong reactivity for the perinatal antigens. In adult ID, light and electron microscopic immunocytochemical analysis showed that most cells had either C or B1 reactivity, a few had either C and D or B1 and D reactivities, and some cells were unreactive for all of the perinatal proteins. Occasional clusters of "adult" acini, however, were strongly positive for B1 and for D, and these clusters were negative for a typical adult acinar marker, the glutamine/glutamic acid-rich proteins (GRP). Also seen in some preparations were a few anomalous acini with the histological appearance of sublingual (SLG) acini. These were negative for the perinatal and adult submandibular gland marker proteins but reactive with an antibody against SLG mucin. We suggest that the B1-positive acini in the adult SMG consist of newly differentiated replacement cells that have arisen from the ID, and that the anomalous mucous acini are, phenotypically, SLG acini that have differentiated within the SMG parenchyma.
在大鼠围产期下颌下腺（SMG）中，I型细胞分泌蛋白C（89 KD），III型细胞分泌B1免疫反应性蛋白（20 - 30 KD）；两种细胞类型均分泌蛋白D（175 KD）。在成熟腺泡中围产期两种细胞类型消失后，仅闰管（ID）细胞对围产期抗原显示强反应性。在成年ID中，光镜和电镜免疫细胞化学分析表明，大多数细胞具有C或B1反应性，少数细胞具有C和D或B1和D反应性，一些细胞对所有围产期蛋白均无反应性。然而，偶尔的“成年”腺泡簇对B1和D呈强阳性，且这些簇对典型的成年腺泡标记物富含谷氨酰胺/谷氨酸的蛋白（GRP）呈阴性。在一些标本中还可见少数具有舌下腺（SLG）腺泡组织学外观的异常腺泡。这些腺泡对围产期和成年下颌下腺标记蛋白呈阴性，但与抗SLG粘蛋白抗体反应。我们认为成年SMG中B1阳性腺泡由源自ID的新分化替代细胞组成，且异常粘液腺泡在表型上是在SMG实质内分化的SLG腺泡。
