Nizet V, Colina K F, Almquist J R, Rubens C E, Smith A L
Division of Infectious Disease, Children's Hospital and Medical Center, Seattle, WA 98105, USA.
J Infect Dis. 1996 Jan;173(1):180-6. doi: 10.1093/infdis/173.1.180.
Nontypeable Haemophilus influenzae strain INT1 was isolated from the blood of a young child with clinical signs of meningitis following acute otitis media. No immunologic or anatomic predisposition of this child for invasive bacterial infection with an unusual organism was documented. Sensitive ELISA proved the absence of intra- or extracellular capsular polysaccharide production by INT1 and Southern blot analysis confirmed the lack of an intact capsulation (cap) gene locus within the chromosome. Nevertheless, INT1 established bacteremia and meningitis in infant and weanling rat models of invasive H. influenzae infection. High-molecular-weight DNA isolated from INT1 was shown to confer an invasive phenotype on transformation of a nonencapsulated, avirulent laboratory strain of H. influenzae. Together these findings imply the presence of one or more as-yet-undiscovered, noncapsular virulence factors of H. influenzae that are capable of mediating invasive disease and resistance to immunologic clearance.
不可分型流感嗜血杆菌INT1菌株是从一名患有急性中耳炎后出现脑膜炎临床症状的幼儿血液中分离出来的。该儿童没有因感染不寻常病原体而发生侵袭性细菌感染的免疫或解剖学易感性记录。灵敏的ELISA证明INT1不产生细胞内或细胞外荚膜多糖，Southern印迹分析证实染色体中缺乏完整的荚膜（cap）基因座。然而，INT1在侵袭性流感嗜血杆菌感染的幼鼠和断奶大鼠模型中引发了菌血症和脑膜炎。从INT1分离的高分子量DNA在转化无荚膜、无毒力的流感嗜血杆菌实验室菌株时显示出赋予侵袭表型。这些发现共同表明存在一种或多种尚未发现的流感嗜血杆菌非荚膜毒力因子，它们能够介导侵袭性疾病并抵抗免疫清除。
