Oldenbourg R, Mei G
Marine Biological Laboratory, Woods Hole, MA 02543, USA.
J Microsc. 1995 Nov;180(Pt 2):140-7. doi: 10.1111/j.1365-2818.1995.tb03669.x.
A new type of polarized light microscope ('new pol-scope') for fast and orientation-independent measurement of birefringent fine structure has been developed. The design of the new pol-scope incorporates a precision universal compensator made from two liquid crystal variable retarders. A video camera and digital image processing system provide fast measurements of specimen anisotropy (retardance magnitude and azimuth) at all points of the image forming the field of view. The images document fine structural and molecular organization within a thin optical section of the specimen. The sensitivity of the current instrument is 0.1 nm of specimen retardance, measured with data gathered in 0.43 s at all 640 x 480 image points. Examples of birefringence measurements in biological (microtubule arrays) and industrial (magneto-optical disc substrate) specimens are presented.
已开发出一种新型偏光显微镜（“新型偏振仪”），用于快速且与取向无关地测量双折射精细结构。新型偏振仪的设计包含一个由两个液晶可变延迟器制成的精密通用补偿器。一台摄像机和数字图像处理系统可对构成视场的图像的所有点处的样品各向异性（延迟量和方位角）进行快速测量。这些图像记录了样品薄光学切片内的精细结构和分子组织。当前仪器的灵敏度为样品延迟0.1纳米，这是通过在0.43秒内收集所有640×480图像点的数据测得的。文中给出了在生物（微管阵列）和工业（磁光盘基板）样品中进行双折射测量的示例。
