Ishizaka A, Hasegawa N, Sayama K, Urano T, Sakamaki F, Nakamura H, Tasaka S, Kanazawa M
Department of Medicine, School of Medicine, Keio University, Tokyo, Japan.
Nihon Kyobu Shikkan Gakkai Zasshi. 1995 Sep;33(9):930-5.
To study the contribution of phagocytosis to the development of acute lung injury, latex particles (2 x 10(9)/kg; mean diameter, 2.84 microns) were injected intravenously or intra-arterially into guinea pigs. 125I-labelled albumin was injected to estimate the degree of lung injury, and 51Cr-labelled red blood cells were injected to correct for blood contamination in the samples. A control group was given saline. Four hours after the injections, the animals were killed, bronchoalveolar lavage was done, and the lungs were examined histopathologically. Animals that had received intravenous and intra-arterial injections of latex particles had more lung water and more pulmonary albumin leakage than animals that had received saline. Histopathological examination revealed massive accumulation of latex in the reticuloendothelial system. These findings suggest that the phagocytic process in the reticuloendothelial system plays a role in the development of lung injury in guinea pigs.
为研究吞噬作用在急性肺损伤发展中的作用，将乳胶颗粒（2×10⁹/kg；平均直径2.84微米）经静脉或动脉注射到豚鼠体内。注射¹²⁵I标记的白蛋白以评估肺损伤程度，注射⁵¹Cr标记的红细胞以校正样本中的血液污染。对照组给予生理盐水。注射后4小时，处死动物，进行支气管肺泡灌洗，并对肺进行组织病理学检查。接受静脉和动脉注射乳胶颗粒的动物比接受生理盐水的动物有更多的肺内水分和更多的肺白蛋白渗漏。组织病理学检查显示，乳胶在网状内皮系统中大量积聚。这些发现表明，网状内皮系统中的吞噬过程在豚鼠肺损伤的发展中起作用。
