Ishizaka A, Sakamaki F, Sayama K, Urano T, Nakamura H, Terashima T, Waki Y, Soejima K, Tasaka S, Kanazawa M
Department of Medicine, School of Medicine, Keio University, Tokyo, Japan.
Nihon Kyobu Shikkan Gakkai Zasshi. 1996 Jun;34(6):665-70.
To assess the effects of 2-chloroadenosine (2CA) on acute lung injury caused by endotoxin (lipopolysaccharide), guinea pigs were given 2CA intravenously. Three groups were used: saline control, endotoxin control and 2CA+ endotoxin. In the endotoxin and 2CA+ endotoxin groups, neutrophils accumulated in bronchoalveolar lavage fluid and in lung tissue. However, neutrophil accumulation did not differ significantly between the endotoxin and the 2CA+ endotoxin groups. The number of macrophages in bronchoalveolar lavage fluid was significantly higher in the endotoxin group than in the saline control group, but the difference between the saline control and the 2CA+ endotoxin groups was not significant. The lung wet-dry weight ratio and 125I-albumin lung tissue-plasma ratio, which were used to measure acute lung injury, were significantly higher in the endotoxin group than in the 2CA+ endotoxin and the saline control groups. However, these ratios did not differ between the 2CA+ endotoxin and the saline control groups. These results suggest that 2CA attenuated endotoxin induced acute lung injury in guinea pigs.
为评估2-氯腺苷（2CA）对由内毒素（脂多糖）引起的急性肺损伤的影响，对豚鼠进行静脉注射2CA。实验分为三组：生理盐水对照组、内毒素对照组和2CA+内毒素组。在内毒素组和2CA+内毒素组中，中性粒细胞在支气管肺泡灌洗液和肺组织中积聚。然而，内毒素组和2CA+内毒素组之间中性粒细胞的积聚没有显著差异。支气管肺泡灌洗液中巨噬细胞的数量在内毒素组中显著高于生理盐水对照组，但生理盐水对照组和2CA+内毒素组之间的差异不显著。用于测量急性肺损伤的肺湿重与干重之比以及125I-白蛋白肺组织与血浆之比在内毒素组中显著高于2CA+内毒素组和生理盐水对照组。然而，2CA+内毒素组和生理盐水对照组之间的这些比值没有差异。这些结果表明，2CA减轻了豚鼠内毒素诱导的急性肺损伤。
