Karr B P, Bubak P J, Sprugel K H, Pavlin E G, Engrav L H
Division of Plastic Surgery, Harborview Medical Center, University of Washington, Seattle, USA.
J Surg Res. 1995 Dec;59(6):739-42. doi: 10.1006/jsre.1995.1232.
This experiment was undertaken for three purposes: (1) to determine a dose-response curve of acute steroid inhibition of wound contraction in the rat; (2) to confirm the results of our preliminary study that platelet-derived growth factor (PDGF) enhanced wound contraction in acutely steroid impaired rats; and (3) to examine the histology of the PDGF-treated wounds. To determine the dose-response of acute steroid inhibition of wound contraction, the rats were suppressed with daily doses of methylprednisolone and wound contraction was measured. Results demonstrated that significant glucocorticoid-induced inhibition of wound contraction begins with daily methylprednisolone doses of 2.0 mg/wound/day or 6.7 mg/kg/day. In an effort to confirm the results of our previous study of the effect of PDGF on wound contraction in acutely steroid-impaired rats and to study the histology of the PDGF-treated wounds, rats were suppressed with methylprednisolone or hydrocortisone and administered daily topical doses of rPDGF-BB. Wound contraction measurements revealed no improvement in the amount or rate of wound contraction. Histologically, the wounds were all very similar in the patterns of cellularity, granulation tissue maturity, collagen content, and epithelial migration. We have clarified the dose response of acute steroid inhibition of wound contraction in rats, data previously unavailable, and have concluded that PDGF in reasonable doses does not improve wound contraction in steroid-impaired rats nor does it alter the histology of the wounds.
（1）确定大鼠急性类固醇抑制伤口收缩的剂量反应曲线；（2）证实我们初步研究的结果，即血小板衍生生长因子（PDGF）可增强急性类固醇损伤大鼠的伤口收缩；（3）检查经PDGF处理的伤口的组织学情况。为了确定急性类固醇抑制伤口收缩的剂量反应，给大鼠每日注射甲基强的松龙以抑制其生理机能，并测量伤口收缩情况。结果表明，当甲基强的松龙的每日剂量达到2.0毫克/伤口/天或6.7毫克/千克/天时，糖皮质激素会对伤口收缩产生显著抑制作用。为了证实我们之前关于PDGF对急性类固醇损伤大鼠伤口收缩影响的研究结果，并研究经PDGF处理的伤口的组织学情况，给大鼠注射甲基强的松龙或氢化可的松，并每日局部给予重组人血小板衍生生长因子-BB（rPDGF-BB）。伤口收缩测量结果显示，伤口收缩的量或速率均未得到改善。从组织学上看，这些伤口在细胞密度、肉芽组织成熟度、胶原蛋白含量和上皮迁移模式方面都非常相似。我们已经明确了大鼠急性类固醇抑制伤口收缩的剂量反应，这是之前未有的数据，并得出结论：合理剂量的PDGF并不能改善类固醇损伤大鼠的伤口收缩，也不会改变伤口的组织学情况。
