在着色性干皮病E组细胞中分离出一种编码紫外线损伤DNA结合因子缺陷的cDNA。

Isolation of a cDNA encoding a UV-damaged DNA binding factor defective in xeroderma pigmentosum group E cells.

作者信息

Hwang B J, Liao J C, Chu G

机构信息

Department of Medicine, Stanford University Medical Center, CA 94305, USA.

出版信息

Mutat Res. 1996 Jan 2;362(1):105-17. doi: 10.1016/0921-8777(95)00040-2.

DOI:10.1016/0921-8777(95)00040-2

PMID:8538642

Abstract

XPE binding factor (XPE-BF) is deficient in a subset of patients from xeroderma pigmentosum complementation group E. Binding activity copurifies with a 125 kDa polypeptide (p125) that binds to DNA damaged by ultraviolet (UV) radiation and many other agents. We isolated cDNA encoding a polypeptide with a predicted amino acid sequence that matched the sequences of eleven tryptic peptides derived from digestion of XPE-BF purified from human placenta. In vitro transcription and translation of the cDNA yielded a polypeptide of 125 kDa that bound specifically to UV-damaged DNA. Therefore the cDNA encodes either all or the major component of XPE-BF.

摘要

着色性干皮病E互补组的一部分患者缺乏XPE结合因子（XPE-BF）。其结合活性与一种125 kDa的多肽（p125）共同纯化，该多肽可与紫外线（UV）辐射及许多其他因素损伤的DNA结合。我们分离出了编码一种多肽的cDNA，其预测的氨基酸序列与源自从人胎盘中纯化的XPE-BF消化产物的11个胰蛋白酶肽段的序列相匹配。该cDNA的体外转录和翻译产生了一种125 kDa的多肽，它能特异性地结合紫外线损伤的DNA。因此，该cDNA编码XPE-BF的全部或主要成分。