RecA蛋白的组成性增加：其对嘧啶二聚体切除及紫外线照射的大肠杆菌存活的影响

Constitutive increase of RecA protein: its influence on pyrimidine dimer excision and survival of UV-irradiated Escherichia coli.

作者信息

Slezáriková V, Masek F, Fridrichová I, Pirsel M, Sedliaková M

机构信息

Department of Molecular Genetics, Slovak Academy of Sciences, Bratislava, Slovakia.

出版信息

Mutat Res. 1996 Jan 2;362(1):61-4. doi: 10.1016/0921-8777(95)00033-x.

DOI:10.1016/0921-8777(95)00033-x

PMID:8538649

Abstract

Transformation of E. coli with the plasmid pRA containing recA gene increased the constitutive level of RecA protein 50-67 fold. This slightly inhibited pyrimidine dimer excision and reduced cell survival in three investigated, UV-irradiated E. coli strains. Our data support the view that RecA protein prematurely present at a high level may mask the dimers. The masking subsequently reduces the dimer excision and switches off the inducing signal.

摘要

用含有recA基因的质粒pRA转化大肠杆菌，可使RecA蛋白的组成型水平提高50至67倍。这在三种经紫外线照射的大肠杆菌菌株中，对嘧啶二聚体切除有轻微抑制作用，并降低了细胞存活率。我们的数据支持这样一种观点，即高水平过早出现的RecA蛋白可能会掩盖二聚体。这种掩盖随后会减少二聚体切除，并关闭诱导信号。