Saika S, Kobata S, Hashizume N, Yamanaka O, Okada Y, Ooshima A
Department of Ophthalmology, Wakayama Medical College, Japan.
Ophthalmic Res. 1995;27(3):129-35. doi: 10.1159/000267645.
To investigate the changes in the corneal epithelial basement membrane following an alkali burn, we examined the immunolocalization of type IV collagen and laminin in the eye of the guinea pig burned with alkali. The burn damaged the corneal, limbal and conjunctival epithelium. After regeneration, basement membrane was interrupted, as indicated by laminin immunoreactivity. Type IV collagen immunoreactivity was transiently expressed in the early healing phase in the epithelial derived from both the cornea and conjunctiva, but was not seen in the normal corneal epithelial basement membrane. Later in the healing process, following transdifferentiation of the conjunctival epithelium into a cornea-like epithelium, its type IV collagen immunoreactivity was weaker than that in the basement membrane of the nontransdifferentiated epithelium. Conjunctival transdifferentiation during healing may have led to transient development of type IV collagen immunoreactivity.
为了研究碱烧伤后角膜上皮基底膜的变化，我们检测了用碱烧伤的豚鼠眼中IV型胶原和层粘连蛋白的免疫定位。烧伤损伤了角膜、角膜缘和结膜上皮。再生后，如层粘连蛋白免疫反应性所示，基底膜中断。IV型胶原免疫反应性在角膜和结膜来源的上皮早期愈合阶段短暂表达，但在正常角膜上皮基底膜中未见。在愈合过程后期，结膜上皮转分化为角膜样上皮后，其IV型胶原免疫反应性比未转分化上皮的基底膜弱。愈合过程中的结膜转分化可能导致了IV型胶原免疫反应性的短暂发展。
