Saika S, Kobata S, Hashizume N, Okada Y, Yamanaka O
Department of Ophthalmology, Wakayama Medical College, Japan.
Cornea. 1993 Sep;12(5):383-90. doi: 10.1097/00003226-199309000-00003.
We investigated the altered immunolocalization of the components of epithelial basement membrane (BM) in rats with alkali burns of the cornea. Type IV collagen, laminin, and fibronectin were immunohistochemically stained in the alkali-burned corneas after various intervals. Linear laminin immunoreactivity, which represented the localization of the BM, was interrupted, probably by epithelial enzymes, under the regenerating epithelium after 24 h. A normal configuration was restored on day 7, when the central corneal BM showed a type IV collagen immunoreactivity that was not detected in BM of normal cornea. The exact cause of the development of type IV collagen immunoreactivity in the healing BM was not determined. Laminin, type IV collagen, and fibronectin, which were not detected in normal stroma, were all detected in the healing corneal stroma with repopulated keratocytes. Moreover, the BM zone under the regenerating epithelium showed fibronectin immunoreactivity on days 5-14. Distribution of these components of BM seemed to be normal 2 months postburn. The alteration of BM components is thought to be an important marker of the healing process in corneas burned with alkali.
我们研究了角膜碱烧伤大鼠上皮基底膜（BM）成分的免疫定位变化。在不同时间间隔后，对碱烧伤角膜中的IV型胶原、层粘连蛋白和纤连蛋白进行免疫组织化学染色。代表BM定位的线性层粘连蛋白免疫反应性在24小时后，可能被上皮酶中断，位于再生上皮下方。在第7天恢复正常结构，此时中央角膜BM显示出正常角膜BM中未检测到的IV型胶原免疫反应性。愈合BM中IV型胶原免疫反应性产生的确切原因尚未确定。在正常基质中未检测到的层粘连蛋白、IV型胶原和纤连蛋白，在有重新填充的角膜细胞的愈合角膜基质中均被检测到。此外，在第5 - 14天，再生上皮下方的BM区显示出纤连蛋白免疫反应性。烧伤后2个月，这些BM成分的分布似乎正常。BM成分的改变被认为是角膜碱烧伤愈合过程的一个重要标志。
