黑曲霉植酸酶在转基因烟草叶片中的稳定积累。
Stable accumulation of Aspergillus niger phytase in transgenic tobacco leaves.
作者信息
Verwoerd T C, van Paridon P A, van Ooyen A J, van Lent J W, Hoekema A, Pen J
机构信息
MOGEN International N.V., Leiden, The Netherlands.
出版信息
Plant Physiol. 1995 Dec;109(4):1199-205. doi: 10.1104/pp.109.4.1199.
Abstract
Phytase from Aspergillus niger increases the availability of phosphorus from feed for monogastric animals by releasing phosphate from the substrate phytic acid. A phytase cDNA was constitutively expressed in transgenic tobacco (Nicotiana tabacum) plants. Secretion of the protein to the extracellular fluid was established by use of the signal sequence from the tobacco pathogen-related protein S. The specific phytase activity in isolated extracellular fluid was found to be approximately 90-fold higher than in total leaf extract, showing that the enzyme was secreted. This was confirmed by use of immunolocalization. Despite differences in glycosylation, specific activities of tobacco and Aspergillus phytase were identical. Phytase was found to be biologically active and to accumulate in leaves up to 14.4% of total soluble protein during plant maturation. Comparison of phytase accumulation and relative mRNA levels showed that phytase stably accumulated in transgenic leaves during plant growth.
摘要
黑曲霉植酸酶通过从底物植酸中释放磷酸盐来提高单胃动物饲料中磷的利用率。一种植酸酶cDNA在转基因烟草（烟草）植株中组成型表达。利用烟草病程相关蛋白S的信号序列将该蛋白分泌到细胞外液中。在分离的细胞外液中发现的特定植酸酶活性比总叶提取物中的活性高约90倍，表明该酶已被分泌。这通过免疫定位得到了证实。尽管糖基化存在差异，但烟草和黑曲霉植酸酶的比活性相同。在植物成熟过程中，发现植酸酶具有生物活性，并在叶片中积累，占总可溶性蛋白的14.4%。植酸酶积累与相对mRNA水平的比较表明，在植物生长过程中，植酸酶在转基因叶片中稳定积累。
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