挥发性茉莉酸甲酯对烟草、黄瓜和拟南芥中氧化脂途径的影响。
Effect of volatile methyl jasmonate on the oxylipin pathway in tobacco, cucumber, and arabidopsis.
作者信息
Avdiushko S, Croft K P, Brown G C, Jackson D M, Hamilton-Kemp T R, Hildebrand D
机构信息
Department of Agronomy, University of Kentucky, Lexington 40546, USA.
出版信息
Plant Physiol. 1995 Dec;109(4):1227-30. doi: 10.1104/pp.109.4.1227.
The effect of atmospheric methyl jasmonate on the oxylipin pathway was investigated in leaves of tobacco (Nicotiana tabacum L.), cucumber (Cucumis sativa L.), and Arabidopsis thaliana (L.). Differential sensitivities of test plants to methyl jasmonate were observed. Thus, different concentrations of methyl jasmonate were required for induction of changes in the oxylipin pathway. Arabidopsis was the least and cucumber the most sensitive to methyl jasmonate. Methyl jasmonate induced the accumulation of lipoxygenase protein and a corresponding increase in extractable lipoxygenase activity. Atmospheric methyl jasmonate additionally induced hydroperoxide lyase activity and the enhanced production of several volatile six-carbon products. It is interesting that lipid hydroperoxidase activity, which is a measure of hydroperoxide lyase plus allene oxide synthase plus possibly other lipid hydroperoxide-metabolizing activities, was not changed by methyl jasmonate treatment. Methyl jasmonate selectively altered the activity of certain enzymes of the oxylipin pathway (lipoxygenase and hydroperoxide lyase) and increased the potential of leaves for greatly enhanced six-carbon-volatile production.
研究了大气中的茉莉酸甲酯对烟草（Nicotiana tabacum L.）、黄瓜（Cucumis sativa L.）和拟南芥（Arabidopsis thaliana (L.)）叶片中氧化脂质途径的影响。观察到受试植物对茉莉酸甲酯的敏感性存在差异。因此，诱导氧化脂质途径变化需要不同浓度的茉莉酸甲酯。拟南芥对茉莉酸甲酯最不敏感，黄瓜最敏感。茉莉酸甲酯诱导脂氧合酶蛋白积累，并使可提取的脂氧合酶活性相应增加。大气中的茉莉酸甲酯还诱导了氢过氧化物裂解酶活性，并增加了几种挥发性六碳产物的生成。有趣的是，脂质氢过氧化物酶活性（衡量氢过氧化物裂解酶、丙二烯氧化物合酶以及可能的其他脂质氢过氧化物代谢活性的指标）并未因茉莉酸甲酯处理而改变。茉莉酸甲酯选择性地改变了氧化脂质途径中某些酶（脂氧合酶和氢过氧化物裂解酶）的活性，并增加了叶片大幅提高六碳挥发性物质生成的潜力。
Zotero 插件