Grimes H D, Koetje D S, Franceschi V R
Department of Botany, Washington State University, Pullman, Washington 99164-4238.
Plant Physiol. 1992 Sep;100(1):433-43. doi: 10.1104/pp.100.1.433.
Exposure of soybean (Glycine max) seedlings to low levels of atmospheric methyl jasmonate induced the expression and accumulation of one or more lipoxygenase(s) in the primary leaves, hypocotyls, epicotyls, and cotyledons. In the primary leaf, the major site of lipoxygenase accumulation in response to methyl jasmonate was in the vacuoles of paraveinal mesophyll cells. In the other organs, however, most of the methyl jasmonate-responsive lipoxygenase(s) were associated with both the epidermal and cortical cells and were present in both vacuoles and plastids. In plastids, the methyl jasmonate-responsive lipoxygenase was sequestered into protein inclusion bodies; no lipoxygenase was evident in either the thylakoids or the stroma. Both spectrophotometric measurement of conjugated diene formation and thin layer chromatography of lipoxygenase product formation indicated that methyl jasmonate caused an increase in the amount of lipoxygenase activity. Electron microscopy of the methyl jasmonate-responsive lipoxygenase protein in the vacuoles showed that it was arranged into a stellate, paracrystalline structure in various cell types other than the paraveinal mesophyll cells. The paracrystals appeared to be composed of tubular elements of between 5 and 8 nm in diameter, were of variable length, and were observed in most cell types of the seedling organs.
将大豆（Glycine max）幼苗暴露于低水平的大气茉莉酸甲酯中，会诱导一片叶、下胚轴、上胚轴和子叶中一种或多种脂氧合酶的表达和积累。在一片叶中，响应茉莉酸甲酯而积累脂氧合酶的主要部位是脉间叶肉细胞的液泡。然而，在其他器官中，大多数响应茉莉酸甲酯的脂氧合酶与表皮细胞和皮层细胞都有关联，并且存在于液泡和质体中。在质体中，响应茉莉酸甲酯的脂氧合酶被隔离到蛋白质包涵体中；在类囊体或基质中均未发现明显的脂氧合酶。共轭二烯形成的分光光度测量和脂氧合酶产物形成的薄层色谱分析均表明，茉莉酸甲酯导致脂氧合酶活性量增加。对液泡中响应茉莉酸甲酯的脂氧合酶蛋白进行电子显微镜观察发现，除脉间叶肉细胞外，它在各种细胞类型中排列成星状、准晶体结构。准晶体似乎由直径在5至8纳米之间的管状元件组成，长度可变，并且在幼苗器官的大多数细胞类型中都能观察到。
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