Yamada N, Shioya N, Kuroyanagi Y
Department of Plastic and Reconstructive Surgery, School of Medicine Kitasato University, Kanagawa, Japan.
Scand J Plast Reconstr Surg Hand Surg. 1995 Sep;29(3):211-9. doi: 10.3109/02844319509050129.
The purpose of this study was to examine whether epithelialisation is promoted when an allogeneic cultured dermal substitute is used as a biological wound dressing. The dermal substitute was prepared by plating fibroblasts on to a spongy collagen matrix, and then culturing them for 7 to 10 days. A new animal wound model was designed to measure re-epithelialisation quantitatively. A full thickness skin defect was made on the dorsum of each of 33 rats; the skin was excised, leaving a layer of pannicular carnosus with an island of intact skin in the central portion of the skin defect. In the first group of rats (n = 13), a piece of cultured dermal substitute was applied to the wound, and a medicated covering material was placed over it. Re-epithelialisation from the island of intact skin was monitored over a period of 7 days. In the second group of rats (n = 10), the wound was covered with an acellular collagen matrix in conjunction with the medicated covering material, and in the third group of rats (n = 10), the wound was covered with the medicated covering material alone. Both the macroscopic and histological findings indicated that the epithelial migration of the first group of rats was far more rapid than that in the other two groups. It comes to the conclusion that the application of this new fibroblastic cultured dermal substitute provided a good environment for the promotion of wound healing.
本研究的目的是检验当使用同种异体培养的真皮替代物作为生物伤口敷料时，是否能促进上皮形成。真皮替代物是通过将成纤维细胞接种到海绵状胶原基质上，然后培养7至10天制备而成。设计了一种新的动物伤口模型来定量测量再上皮形成。在33只大鼠的背部制造全层皮肤缺损；切除皮肤，在皮肤缺损的中央部分留下一层 pannicular carnosus 和一块完整皮肤岛。在第一组大鼠（n = 13）中，将一块培养的真皮替代物应用于伤口，并在其上放置一种含药覆盖材料。在7天的时间内监测来自完整皮肤岛的再上皮形成情况。在第二组大鼠（n = 10）中，伤口用无细胞胶原基质结合含药覆盖材料覆盖，在第三组大鼠（n = 10）中，伤口仅用含药覆盖材料覆盖。宏观和组织学结果均表明，第一组大鼠的上皮迁移远比其他两组迅速。得出的结论是，这种新的成纤维细胞培养真皮替代物的应用为促进伤口愈合提供了良好的环境。
