Danel C, Erzurum S C, McElvaney N G, Crystal R G
National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA.
Am J Respir Crit Care Med. 1996 Jan;153(1):362-8. doi: 10.1164/ajrccm.153.1.8542144.
Nasal and bronchial brushings and bronchial biopsies were evaluated from patients with cystic fibrosis (CF) and from normal subjects to quantify epithelial and inflammatory cell types. Epithelial in both groups were dominated by ciliated cells. The relative proportions of epithelial cells recovered by brushing and biopsy were similar, but with more basal cells from biopsies than from brushings. In nasal brushings, the numbers and percentages of epithelial subtypes were similar in both groups. In bronchial brushings, the number of recovered cells was 2.5-fold increased in the CF group compared with that in the normal group because of large numbers of neutrophils. The proportion of ciliated cells was lower in the CF group than in the normal group. Thus, even though the CF transmembrane conductance regulator mutations are expressed similarly in the nasal and bronchial epithelium in CF, the consequences are different, with little inflammation and no changes in the proportions of epithelial cells in the nasal epithelium, compared with marked neutrophil inflammation on the epithelial surface and significant changes in epithelial populations in the large airways. Airway brushing permits repetitive evaluation of the airway epithelium in CF, a useful methodology in the assessment of new therapies for this disorder.
对囊性纤维化(CF)患者和正常受试者的鼻腔及支气管刷检样本和支气管活检样本进行评估,以量化上皮细胞和炎症细胞类型。两组的上皮细胞均以纤毛细胞为主。通过刷检和活检回收的上皮细胞相对比例相似,但活检样本中的基底细胞比刷检样本中的更多。在鼻腔刷检中,两组上皮细胞亚型的数量和百分比相似。在支气管刷检中,由于大量中性粒细胞,CF组回收的细胞数量比正常组增加了2.5倍。CF组纤毛细胞的比例低于正常组。因此,尽管CF跨膜电导调节因子突变在CF患者的鼻腔和支气管上皮中表达相似,但其后果却不同,鼻腔上皮几乎没有炎症且上皮细胞比例没有变化,而大气道上皮表面有明显的中性粒细胞炎症且上皮细胞群体有显著变化。气道刷检可对CF患者的气道上皮进行重复评估,这是评估该疾病新疗法的一种有用方法。