Yang Y H, Cho J S, Min H W, Lee C H, Song C H
Department of Obstetrics and Gynecology, Yonsei University College of Medicine, Seoul, Korea.
J Obstet Gynaecol (Tokyo 1995). 1995 Oct;21(5):443-50. doi: 10.1111/j.1447-0756.1995.tb01035.x.
To determine whether abnormally collected fetal body fluid could be used as alternative cell sources for cytogenetic assessment.
Using modified lymphocyte culture, the abnormal fetal body fluids obtained from the five fetal anomaly cases (3 cystic hygromas, 1 hydrothorax, and 1 isolated ascites) were analyzed in order to determine their karyotypes.
Two cases showed 45, X and 46, XX/45, X and the other three showed a normal karyotype.
The cytogenetic analysis from body fluid aspiration yields a rapid and accurate result within 96 hours. This method could be helpful when cordocentesis or placental biopsy is not feasible or when time requirement for amniotic fluid cell culture is burdensome to patient management.
确定异常采集的胎儿体液是否可作为细胞遗传学评估的替代细胞来源。
采用改良淋巴细胞培养法，对从5例胎儿异常病例（3例囊状水瘤、1例胸腔积液和1例单纯腹水）获取的异常胎儿体液进行分析，以确定其核型。
2例显示为45,X和46,XX/45,X，另外3例显示核型正常。
从抽取的体液进行细胞遗传学分析可在96小时内得出快速准确的结果。当脐血穿刺或胎盘活检不可行或羊水细胞培养的时间要求对患者管理造成负担时，该方法可能会有所帮助。
