从以木材水解物为培养基生长的假单胞菌属31260中生产琥珀酰聚糖及其流变学性质
Production and rheological properties of a succinoglycan from Pseudomonas sp. 31260 grown on wood hydrolysates.
作者信息
Meade M J, Tanenbaum S W, Nakas J P
机构信息
Department of Environmental and Forest Biology, State University of New York College of Environmental Science and Forestry, Syracuse 13210, USA.
出版信息
Can J Microbiol. 1995 Dec;41(12):1147-52. doi: 10.1139/m95-160.
Pseudomonas sp. ATCC 31260 produced substantial amounts of anionic extracellular polysaccharide (EPS) from a mineral acid hydrolysate of wood, prepared using the "Tennessee Valley Authority" process. Partially purified EPS production approached 16.5 g/L (as hexadecyltrimethylammonium bromide precipitate) when the pH of the hydrolysate was initially adjusted to 7.5 and amended with 0.05% each of peptone and yeast extract. This EPS, now characterized as a succinoglycan, is composed of glucose, galactose, succinate, pyruvate, and acetate. Solutions of this EPS are pseudoplastic, and under specified conditions, are rheologically comparable with commercially available xanthan.
铜绿假单胞菌ATCC 31260利用“田纳西河谷管理局”工艺制备的木材无机酸水解产物，能产生大量阴离子胞外多糖（EPS）。当初始将水解产物的pH值调至7.5，并添加0.05%的蛋白胨和酵母提取物时，部分纯化的EPS产量接近16.5 g/L（以十六烷基三甲基溴化铵沉淀计）。这种现在被鉴定为琥珀聚糖的EPS由葡萄糖、半乳糖、琥珀酸、丙酮酸和乙酸组成。该EPS溶液为假塑性流体，在特定条件下，其流变学性质与市售黄原胶相当。
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