急性淋巴细胞白血病中髓系细胞系的参与：一项形态学、抗体、染色体（MAC）研究

Myeloid lineage involvement in acute lymphoblastic leukemia: a morphology antibody chromosomes (MAC) study.

作者信息

Larramendy M, el-Rifai W, Saarinen U, Alitalo R, Luomahaara S, Knuutila S

机构信息

Department of Medical Genetics, University of Helsinki, Finland.

出版信息

Exp Hematol. 1995 Dec;23(14):1563-7.

PMID:8542947

Abstract

We looked for clonal chromosomal abnormalities in myeloid cell lineages in the bone marrow aspirates from six children with acute lymphoblastic leukemia (ALL). The study was carried out using a combination of MAC (morphology, antibody, chromosomes) and in situ hybridization procedures. In patients whose leukemic cells expressed only lymphoid antigens, we found chromosomal aberrations in CD10- and CD20/22-positive lymphoid cells. Mature CD22+ and CD3+ lymphocytes did not have the chromosomal aberrations. In one patient whose leukemic cells also expressed myeloid-associated antigens, the clonal chromosome aberrations were seen not only in the CD10+ and CD19+ blasts, but also in glycophorin A-positive morphologically nonleukemic erythroblasts.

摘要

我们在6例急性淋巴细胞白血病（ALL）患儿的骨髓穿刺物中寻找髓系细胞谱系中的克隆性染色体异常。该研究采用形态学、抗体、染色体（MAC）和原位杂交程序相结合的方法进行。在白血病细胞仅表达淋巴样抗原的患者中，我们在CD10和CD20/22阳性的淋巴样细胞中发现了染色体畸变。成熟的CD22 +和CD3 +淋巴细胞没有染色体畸变。在1例白血病细胞也表达髓系相关抗原的患者中，不仅在CD10 +和CD19 +母细胞中发现了克隆性染色体畸变，而且在糖蛋白A阳性的形态学上非白血病的成红细胞中也发现了克隆性染色体畸变。