Taylor W R, Chadee D N, Allis C D, Wright J A, Davie J R
Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Canada.
FEBS Lett. 1995 Dec 11;377(1):51-3. doi: 10.1016/0014-5793(95)01314-8.
H1 histones play an important role in regulating higher order structure of chromatin and are potential regulators of gene expression. H1s are phosphorylated, a modification which alters their interaction with DNA. We measured the abundance of three phosphorylated H1 subtypes in mouse fibroblasts transformed by combinations of ras, myc and mutant p53 which differ in metastatic potential. We found that there is an increase in phosphorylation of H1 subtypes in fibroblasts transformed with ras, myc and mutant p53. This increase was found to correlate with cellular transformation but not with induction of the metastatic phenotype.
H1组蛋白在调节染色质的高级结构中发挥重要作用，并且是基因表达的潜在调节因子。H1组蛋白会发生磷酸化，这种修饰改变了它们与DNA的相互作用。我们测定了由具有不同转移潜能的ras、myc和突变型p53组合转化的小鼠成纤维细胞中三种磷酸化H1亚型的丰度。我们发现，用ras、myc和突变型p53转化的成纤维细胞中H1亚型的磷酸化增加。这种增加与细胞转化相关，但与转移表型的诱导无关。
