5-氮杂胞苷对BSC-1细胞胞质核糖体核糖核酸和信使核糖核酸的影响。
Effect of 5-azacytidine on cytoplasmic ribosomal and messenger ribonucleic acids in BSC-1 cells.
作者信息
Reuveni Y, Rosenthal L J
出版信息
Antimicrob Agents Chemother. 1979 Feb;15(2):235-9. doi: 10.1128/AAC.15.2.235.
5-Azacytidine (5-AzaCR) inhibited the accumulation of 28S and 18S ribonucleic acids (RNAs) in the cytoplasm of treated cells. The inhibition of 28S and 18S RNAs in the cytoplasm of BSC-1 cells was dependent upon the concentration of 5-AzaCR employed and the time of exposure. At a concentration of 200 mug/ml for 2 h, 5-AzaCR inhibited the cytoplasmic 28S and 18S RNAs by 80 and 70%, respectively. The 28S and 18S RNAs that appeared in the cytoplasm of treated cultures had no altered secondary structure, as analyzed by polyacrylamide gel electrophoresis. The inhibitory effect on cytoplasmic 28S and 18S RNAs was found to be reversible, and removal of 5-AzaCR from treated cultures allowed the accumulation of cytoplasmic 28S and 18S RNAs to almost normal levels by 20 h. 5-AzaCR appeared to have no effect upon the synthesis and processing of polyadenylic acid-containing messenger RNA in treated cultures. However, the formation of the 80S ribosomal subunit appeared to be inhibited in drug-treated cells. Moreover, 5-AzaCR treatment caused a disaggregation of polyribosomes and an accumulation of 80S ribosomes.
5-氮杂胞苷（5-AzaCR）抑制了处理细胞胞质中28S和18S核糖核酸（RNA）的积累。BSC-1细胞胞质中28S和18S RNA的抑制作用取决于所用5-AzaCR的浓度和暴露时间。在200μg/ml的浓度下处理2小时，5-AzaCR分别使胞质28S和18S RNA的含量降低了80%和70%。经聚丙烯酰胺凝胶电泳分析，处理培养物胞质中出现的28S和18S RNA的二级结构未发生改变。对胞质28S和18S RNA 的抑制作用是可逆的，从处理培养物中去除5-AzaCR后，20小时内胞质28S和18S RNA的积累量几乎恢复到正常水平。5-AzaCR似乎对处理培养物中含聚腺苷酸的信使RNA的合成和加工没有影响。然而，药物处理的细胞中80S核糖体亚基的形成似乎受到了抑制。此外，5-AzaCR处理导致多核糖体解聚和80S核糖体积累。
Zotero 插件